Dataset: Impairment of RNA polymerase I elongation rate induces defects in ribosomal RNA processing and ribosome biogenesis

External ID

GSE196146

Reference

Huffines AK, et al. (2022)

Channels

1

Conditions

6

Description

The objective of this study was to investigate the relationship between Pol I transcription elongation rate and ribosomal RNA processing in vivo by using a yeast strain containing a mutation in Pol I. This mutation, rpa190-F1205H, has been previously characterized to have a reduced elongation rate in vitro. Here, we used native elongating transcript sequencing (NET-seq) to determine the effect of this mutation on Pol I occupancy in vivo. Our findings demonstrate that this mutation induces increased Pol I pausing, especially in the first half of the 35S gene, and causes sequence-specific changes in Pol I occupancy.

Categories

RNA processing and metabolism

GEO