CAN1 / YEL063C Overview
- Standard Name
- CAN1 1
- Systematic Name
- YEL063C
- SGD ID
- SGD:S000000789
- Feature Type
- ORF , Verified
- Description
- Plasma membrane arginine permease; transceptor that senses extracellular basic amino acids; negative regulator of biofilm formation; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication 2 3 4 5 6 7 8 9
- Name Description
- CANavanine resistance 6
- Paralog
- ALP1 7
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- CAN1 is located on the left arm of chromosome V between AVT2 transporter and NPR2 SEACIT complex subunit; coding sequence is 1773 nucleotides long with 3 SNPs, one of which leads to an Ile/Val polymorphism at residue 534; CAN1 has a paralog, ALP1, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Can1p is 590 amino acids long, short-lived, low in abundance; has an amino acid permease domain, undergoes various post-translational modifications including phosphorylation and ubiquitinylation at 12 sites within the N-terminal region
- Length (a.a.)
- 590
- Mol. Weight (Da)
- 65789.8
- Isoelectric Point
- 8.59
- Median Abundance (molecules/cell)
- 3618 +/- 2020
- Half-life (hr)
- 1.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CAN1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Permease involved in transmembrane transport of basic amino acids; localizes to the plasma membrane, eisosome, endoplasmic reticulum, and mitochondria
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in basic amino acid transport (IDA)
- involved in L-arginine transmembrane transport (IGI)
- involved in transmembrane transport (IDA)
Cellular Component
- Manually Curated
- located in eisosome (IDA)
- located in endoplasmic reticulum (HDA)
- located in mitochondrion (HDA)
- located in plasma membrane (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutants are resistant to canavanine, have an increased lifespan, and are unable to take up arginine; overexpression mutants are more resistant to doxorubicin; null mutants in high-scale studies show a decrease in endocytosis, abnormal vacuolar morphology and a decrease in competitive fitness on synthetic complete media
Classical Genetics
- unspecified
- overexpression
- null
- null
- biofilm formation: decreased
- chemical compound accumulation: abnormal
- chronological lifespan: increased
- competitive fitness: decreased
- endocytosis: decreased
- oxidative stress resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- starvation resistance: decreased
- vacuolar morphology: abnormal
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The can1 null mutant is viable; the null mutant of paralog alp1 is viable; the can1 alp1 double mutant is inviable; interacts physically with proteins involved in lipid metabolism, interacts genetically with genes involved in amino acid metabolism
80 total interactions for 77 unique genes
Physical Interactions
- Affinity Capture-MS: 3
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 2
- Co-localization: 2
- PCA: 46
- Protein-RNA: 1
- Two-hybrid: 1
Genetic Interactions
- Dosage Growth Defect: 1
- Phenotypic Enhancement: 1
- Phenotypic Suppression: 2
- Positive Genetic: 2
- Synthetic Growth Defect: 3
- Synthetic Lethality: 9
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 6
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-06-19
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.