CBK1 / YNL161W Overview
- Standard Name
- CBK1 1
- Systematic Name
- YNL161W
- SGD ID
- SGD:S000005105
- Feature Type
- ORF , Verified
- Description
- Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress 2 3 4 5 6 7 8 9 10 11 12 13 14
- Name Description
- Cell wall Biosynthesis Kinase 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 756
- Mol. Weight (Da)
- 86948.0
- Isoelectric Point
- 8.04
- Median Abundance (molecules/cell)
- 2674 +/- 562
- Half-life (hr)
- 8.0
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CBK1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein serine/threonine kinase involved in apical bud growth, establishment or maintenance of cell polarity, regulation of cell wall organization, and cell separation after cytokinesis; localizes to incipient bud sites, sites of polarized growth, the nucleus, and the cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- enables protein serine/threonine kinase activity (IDA)
Biological Process
- Manually Curated
- involved in budding cell apical bud growth (IGI, IMP)
- involved in establishment or maintenance of actin cytoskeleton polarity (IMP)
- involved in establishment or maintenance of cell polarity (IMP)
- involved in regulation of fungal-type cell wall organization (IGI)
- involved in regulation of protein secretion (IMP)
- involved in septum digestion after cytokinesis (IMP)
Cellular Component
- Manually Curated
- located in cell cortex (IDA)
- located in cellular bud (IDA)
- located in cellular bud neck (HDA, IDA)
- located in cellular bud tip (IDA, HDA)
- located in cytoplasm (IDA, HDA)
- located in cytoplasmic stress granule (HDA)
- located in incipient cellular bud site (IDA)
- located in mating projection tip (IDA, HDA)
- located in nucleus (IDA)
- located in prospore membrane (HDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant has defects in actin cytoskeleton and shows abnormal budding pattern, round cell shape, crinkled colony appearance and increased cell aggregation in liquid cultures; null mutant is also defective in mating projection formation; null mutants in Sigma1278b are incapable of invasive growth
Classical Genetics
- null
- actin cytoskeleton morphology: abnormal
- axial budding pattern: decreased
- axial budding pattern: normal
- bipolar budding pattern: decreased
- budding pattern: abnormal
- cell shape: abnormal
- chemical compound accumulation: increased
- colony appearance: abnormal
- innate thermotolerance: increased
- inviable
- liquid culture appearance: abnormal
- mating projection morphology: abnormal
- pheromone sensitivity: decreased
- pheromone-induced cell cycle arrest: normal
- protein activity: decreased
- protein/peptide distribution: abnormal
- RNA accumulation: decreased
- viable
- unspecified
- activation
- reduction of function
- null
- reduction of function
- overexpression
- unspecified
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
246 total interactions for 153 unique genes
Physical Interactions
- Affinity Capture-MS: 66
- Affinity Capture-RNA: 11
- Affinity Capture-Western: 12
- Biochemical Activity: 7
- Co-crystal Structure: 3
- Co-localization: 1
- Co-purification: 2
- PCA: 15
- Protein-peptide: 1
- Reconstituted Complex: 14
- Two-hybrid: 31
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 3
- Dosage Rescue: 16
- Negative Genetic: 22
- Phenotypic Enhancement: 10
- Phenotypic Suppression: 7
- Synthetic Growth Defect: 13
- Synthetic Lethality: 8
- Synthetic Rescue: 3
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.