CDC34 / YDR054C Overview
- Standard Name
- CDC34 1
- Systematic Name
- YDR054C
- SGD ID
- SGD:S000002461
- Aliases
- DNA6 51 52 , UBC3 8
- Feature Type
- ORF , Verified
- Description
- Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress; human CDC34 functionally complements the thermosensitivity of the cdc34-2 mutant 2 3 4
- Name Description
- Cell Division Cycle
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Ubiquitin-conjugating enzyme (E2) catalytic subunit of SCF ubiquitin-protein ligase complex; protein abundance increases in response to DNA replication stress
- Length (a.a.)
- 295
- Mol. Weight (Da)
- 34019.8
- Isoelectric Point
- 3.92
- Median Abundance (molecules/cell)
- 4374 +/- 1541
- Half-life (hr)
- 3.3
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CDC34 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Ubiquitin-conjugating enzyme (E2); subunit of Skp1/Cul1/F-box (SCF) complexes involved in proteasomal-dependent protein catabolism; capable of autoubiquitination; required for both the G1/S and G2/M phase transitions of the mitotic cell cycle
View computational annotations
Molecular Function
- Manually Curated
- contributes to ubiquitin protein ligase activity (IDA)
- contributes to ubiquitin-protein transferase activity (IDA, IMP)
Biological Process
- Manually Curated
- involved in G2/M transition of mitotic cell cycle (IGI)
- involved in protein autoubiquitination (IMP, IDA)
- involved in protein polyubiquitination (IDA)
- involved in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process (IDA)
- involved in ubiquitin-dependent protein catabolic process (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
- SCF-Cdc4 ubiquitin ligase complex
- SCF-Das1 ubiquitin ligase complex
- SCF-Dia2 ubiquitin ligase complex
- SCF-Grr1 ubiquitin ligase complex
- SCF-Hrt3 ubiquitin ligase complex
- SCF-MET30 E3 ubiquitin ligase complex
- SCF-Mdm30 ubiquitin ligase complex
- SCF-Saf1 ubiquitin ligase complex
- SCF-Ufo1 ubiquitin ligase complex
- SCF-Ydr131c ubiquitin ligase complex
- SCF-Ylr352w ubiquitin ligase complex
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; conditional mutants display a cell cycle arrest in the G1 phase with both elongated and and multi-budded cells; mutation results in increased accumulation and decreased ubiquitination of specific proteins, decreased resistance to metals and DNA damaging agents, decreased telomere length, decreased innate thermotolerance and abnormal mitochondrial morphology; overexpression results in decreased growth rate, increased resistance to metals, increased ubiquitination of specific proteins, and abnormal vacuolar morphology
Classical Genetics
- overexpression
- null
- repressible
- conditional
- cell cycle progression in G1 phase: arrested
- cellular morphology: abnormal
- heat sensitivity: increased
- heat sensitivity: normal
- metal resistance: decreased
- protein/peptide accumulation: decreased
- protein/peptide accumulation: increased
- protein/peptide modification: decreased
- resistance to chemicals: decreased
- reduction of function
- dominant negative
- repressible
- null
- reduction of function
- overexpression
- conditional
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast CDC34 is homologous to human UBE2B, and has been used to study congenital heart disease and azoospermia
High-Throughput
- is_implicated_in azoospermia (IGI, ISS)
- is_implicated_in congenital heart disease (IGI, ISS)
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
631 total interactions for 458 unique genes
Physical Interactions
- Affinity Capture-MS: 34
- Affinity Capture-RNA: 7
- Affinity Capture-Western: 24
- Biochemical Activity: 31
- Co-crystal Structure: 3
- Co-fractionation: 2
- FRET: 1
- PCA: 1
- Reconstituted Complex: 41
- Two-hybrid: 1
Genetic Interactions
- Dosage Growth Defect: 4
- Dosage Lethality: 7
- Dosage Rescue: 17
- Negative Genetic: 231
- Phenotypic Enhancement: 5
- Phenotypic Suppression: 7
- Positive Genetic: 65
- Synthetic Growth Defect: 55
- Synthetic Haploinsufficiency: 3
- Synthetic Lethality: 74
- Synthetic Rescue: 18
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 11
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2007-06-14
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.