CLA4 / YNL298W Overview
- Standard Name
- CLA4 1
- Systematic Name
- YNL298W
- SGD ID
- SGD:S000005242
- Aliases
- ERC10 1 4 9
- Feature Type
- ORF , Verified
- Description
- Cdc42p-activated signal transducing kinase; member of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p; CLA4 has a paralog, SKM1, that arose from the whole genome duplication 1 2 3 4 5 7 8
- Name Description
- CLn Activity dependant 6
- Paralog
- SKM1 8
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- CLA4 is located on the left arm of chromosome XIV near the telomere and between TRF5 poly(A) polymerase and MON2 endocytosis protein; coding sequence is 2529 nucleotides long with 12 SNPs, 6 of which cause amino acid polymorphisms, and an in-frame deletion in SK1 that removes Q377; CLA4 has paralog SKM1 from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Cla4p is 842 amino acids long, short-lived, very low in abundance; contains pleckstrin homology domain, CRIB domain, kinase domain; phosphorylated on 53 residues
- Length (a.a.)
- 842
- Mol. Weight (Da)
- 93928.1
- Isoelectric Point
- 9.51
- Median Abundance (molecules/cell)
- 2091 +/- 981
- Half-life (hr)
- 5.8
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CLA4 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Serine/Threonine kinase; involved in septin ring organization, vacuole inheritance, regulation of exit from mitosis, positive regulation of protein import into nucleus; negative regulation of sterol import by negative regulation of transcription from RNA Pol II promoter; localizes to the vacuole, nucleus and bud
View computational annotations
Molecular Function
- Manually Curated
- enables kinase activity (IDA)
- enables protein kinase activity (HDA)
- enables protein serine/threonine kinase activity (IDA)
Biological Process
- Manually Curated
- involved in mitotic cell cycle (IDA)
- involved in negative regulation of gene expression (IMP, IGI)
- involved in negative regulation of sterol import (IMP)
- involved in negative regulation of transcription by RNA polymerase II (IMP)
- involved in positive regulation of protein import into nucleus (IMP)
- involved in regulation of exit from mitosis (IMP)
- involved in response to pheromone (IGI)
- involved in septin ring organization (IMP, IGI)
- involved in sterol import (IMP)
- involved in vacuole inheritance (IMP, IGI)
Cellular Component
- Manually Curated
- located in cellular bud (IDA)
- located in fungal-type vacuole (IDA)
- located in nucleus (IMP)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant is hypersensitive to zymolyase treatment and displays lowered sensitivity to mating pheromones; null mutant accumulates elevated levels of sterol esters under anaerobic conditions; in large-scale studies the null mutant displays slow growth, haploinsufficiency, large cell size, decreased filamentous growth, and sensitivity to various chemicals
Classical Genetics
- null
- anaerobic growth: decreased
- bud morphology: abnormal
- cell size: increased
- chemical compound accumulation: abnormal
- chitin deposition: increased
- competitive fitness: decreased
- filamentous growth: decreased
- haploinsufficient
- heat sensitivity: increased
- hydrostatic pressure resistance: decreased
- invasive growth: absent
- mating response: decreased
- metal resistance: decreased
- metal resistance: increased
- oxidative stress resistance: decreased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- respiratory growth: absent
- utilization of nitrogen source: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Cla4p interacts physically with proteins involved in mitotic cell cycle, CLA4 interacts genetically with genes involved in transcription; the cla4 null mutant is viable, the null mutant of paralog skm1 is viable, the cla4 skm1 double mutant is viable, the cla4 skm1 ste20 triple mutant shows growth defects
1486 total interactions for 754 unique genes
Physical Interactions
- Affinity Capture-MS: 43
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 14
- Biochemical Activity: 43
- Co-localization: 1
- Co-purification: 1
- FRET: 1
- PCA: 6
- Protein-peptide: 4
- Protein-RNA: 1
- Reconstituted Complex: 14
- Two-hybrid: 19
Genetic Interactions
- Dosage Growth Defect: 213
- Dosage Lethality: 7
- Dosage Rescue: 10
- Negative Genetic: 620
- Phenotypic Enhancement: 28
- Phenotypic Suppression: 16
- Positive Genetic: 82
- Synthetic Growth Defect: 124
- Synthetic Lethality: 218
- Synthetic Rescue: 16
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- CLA4 promoter is bound by Hfi1p, Msn2p, Put3p, Reb1p, Rpd3p, Spt3p, Swi4p, Ume6p, and Yap1p in response to heat; CLA4 transcription is regulated by Sfp1p in response to stress, and is downregulated by Ixr1p in reponse to hypoxia; Cla4 protein activity is regulated by protein phosphatase PP2A and Cdc28p; Cla4 protein activity is downregulated by Fus3p during mating
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-07-02
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.