DAL80 / YKR034W Overview
- Standard Name
- DAL80
- Systematic Name
- YKR034W
- SGD ID
- SGD:S000001742
- Aliases
- UGA43 16
- Feature Type
- ORF , Verified
- Description
- Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication 1 2 3 4
- Name Description
- Degradation of Allantoin
- Paralog
- GZF3 4
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- DAL80 has a paralog, GZF3, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 269
- Mol. Weight (Da)
- 30172.9
- Isoelectric Point
- 8.74
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all DAL80 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- A RNA polymerase II transcription factor that binds specific DNA sequence; negatively regulates transcription and is involved in nitrogen catabolite repression of transcription; localized to the nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables DNA-binding transcription factor activity, RNA polymerase II-specific (IDA)
- enables sequence-specific DNA binding (IDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in negative regulation of transcription by RNA polymerase II (IMP)
- involved in nitrogen catabolite repression of transcription (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant displays elevated expression of several genes controlled by nitrogen catabolite repression system; both null mutation and overexpression in strain Sigma1278b cause increased filamentous growth
Classical Genetics
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The dal80 null mutant is viable; the null mutant of paralog gzf3 is viable; the dal80 gzf3 double mutant has not been annotated for phenotype.
108 total interactions for 97 unique genes
Physical Interactions
- Affinity Capture-RNA: 1
- Affinity Capture-Western: 1
- Co-localization: 1
- Reconstituted Complex: 6
- Two-hybrid: 27
Genetic Interactions
- Dosage Rescue: 2
- Negative Genetic: 63
- Positive Genetic: 6
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- DAL80 encodes a transcriptional repressor that is a member of the C4 or GATA class. The repressors Dal80p and Gzf3p and the transcriptional activators Gat1p and Gln3p, all members of the GATA class with homologous zinc fingers, bind to the same core consensus sequence and collectively regulate the nitrogen catabolite repression (NCR) pathway, which controls a set of at least 90 genes required for uptake and utilization of different nitrogen sources. Dal80p exerts its repressive effect by three different mechanisms: repression of the genes encoding the activators Gat1p and Gln3p; competition with Gat1p and Gln3p for binding at the promoters of NCR target genes; and direct repression of NCR target genes. DAL80 transcription is regulated by the NCR pathway, primarily by autoregulation and by Gln3p regulation. In addition, the four GATA factors interact with each other to form homodimers or multiple different heterodimers, allowing for fine-tuning of NCR regulation.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2010-05-17
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.