ERG6 / YML008C Overview
- Standard Name
- ERG6 1
- Systematic Name
- YML008C
- SGD ID
- SGD:S000004467
- Aliases
- VID1 12 , ISE1 , LIS1 7 , SED6
- Feature Type
- ORF , Verified
- Description
- Delta(24)-sterol C-methyltransferase; converts zymosterol to fecosterol in the ergosterol biosynthetic pathway by methylating position C-24; localized to lipid particles, the plasma membrane-associated endoplasmic reticulum, and the mitochondrial outer membrane 1 2 3 4 5
- Name Description
- ERGosterol biosynthesis
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 383
- Mol. Weight (Da)
- 43422.2
- Isoelectric Point
- 5.52
- Median Abundance (molecules/cell)
- 23127 +/- 6001
- Half-life (hr)
- 10.0
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all ERG6 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sterol 24-C-methyltransferase involved in ergosterol biosynthesis; localizes to mitochondria, lipid droplet, and endoplasmic reticulum
View computational annotations
Molecular Function
- Manually Curated
- enables sterol 24-C-methyltransferase activity (IDA)
Biological Process
- Manually Curated
- involved in ergosterol biosynthetic process (IDA, IMP)
Cellular Component
- Manually Curated
- is active in endoplasmic reticulum (IDA)
- located in lipid droplet (IDA)
- located in mitochondrial outer membrane (HDA)
- located in mitochondrion (HDA)
Pathways
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant cannot synthesize ergosterol but accumulates zymosterol; null mutant fails to exhibit lifespan extension under caloric restriction and is sensitive to metals and alkaline pH; overproduction leads to abnormal nuclear and actin cytoskeletal morphology; in large-scale studies, null mutant exhibits reduced competitive fitness and sensitivity to various drugs, but increased resistance to azole antifungal drugs and amphotericin B
Classical Genetics
- reduction of function
- overexpression
- null
- alkaline pH resistance: decreased
- apoptosis: increased
- cell death: increased
- chemical compound accumulation: absent
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- endoplasmic reticulum morphology: abnormal
- hydrostatic pressure resistance: decreased
- hyperosmotic stress resistance: decreased
- mating efficiency: decreased
- metal resistance: decreased
- mitochondrial morphology: abnormal
- nutrient uptake: decreased rate
- oxidative stress resistance: decreased
- protein/peptide accumulation: increased
- replicative lifespan: decreased
- replicative lifespan: normal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- RNA accumulation: increased
- vacuolar morphology: abnormal
- conditional
- unspecified
- null
- acid pH resistance: decreased
- alkaline pH resistance: decreased
- auxotrophy
- chemical compound accumulation: abnormal
- chronological lifespan: increased
- competitive fitness: decreased
- desiccation resistance: decreased
- endocytosis: decreased
- haploinsufficient
- hyperosmotic stress resistance: decreased
- innate thermotolerance: decreased
- metal resistance: decreased
- mitochondrial morphology: abnormal
- oxidative stress resistance: decreased
- protein/peptide distribution: abnormal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- respiratory growth: absent
- spore germination: decreased
- stress resistance: decreased
- toxin resistance: decreased
- transposable element transposition: decreased
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- UV resistance: decreased
- vacuolar morphology: abnormal
- viable
- overexpression
- unspecified
- repressible
- reduction of function
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
1089 total interactions for 641 unique genes
Physical Interactions
- Affinity Capture-MS: 30
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 6
- Co-crystal Structure: 1
- Co-fractionation: 1
- Co-localization: 3
- Co-purification: 1
- PCA: 71
- Proximity Label-MS: 3
- Reconstituted Complex: 3
- Two-hybrid: 7
Genetic Interactions
- Dosage Rescue: 5
- Negative Genetic: 736
- Phenotypic Enhancement: 19
- Phenotypic Suppression: 6
- Positive Genetic: 132
- Synthetic Growth Defect: 27
- Synthetic Lethality: 15
- Synthetic Rescue: 19
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 15
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2000-09-05
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.