ESA1 / YOR244W Overview
- Standard Name
- ESA1 1
- Systematic Name
- YOR244W
- SGD ID
- SGD:S000005770
- Aliases
- TAS1 , KAT5 8
- Feature Type
- ORF , Verified
- Description
- Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; master regulator of cellular acetylation balance; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy; human ortholog TIP60/KAT5 is implicated in cancer and other diseases, functionally complements lethality of the esa1 null mutation 1 2 3 4 5 6 7
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- ESA1/YOR244W is located on the right arm of chromosome XV between PUS7 pseudouridine synthase and DGA1 diacylglycerol acyltransferase; coding sequence is 1338 nucleotides long with 2 SNPs, one of which causes a Gly/Glu polymorphism at residue 5
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Esa1p is 445 amino acids long, longer-lived, low in abundance; contains multiple acetyltransferase domains, acetylated on 4 residues, phosphorylated on 6 residues
- Length (a.a.)
- 445
- Mol. Weight (Da)
- 52614.4
- Isoelectric Point
- 7.82
- Median Abundance (molecules/cell)
- 1772 +/- 610
- Half-life (hr)
- 13.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all ESA1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Acetyltransferase involved in acetylation of histone H4, chromatin silencing, regulation of transcription, macroautophagy, DNA repair and other cellular processes; subunit of the Piccolo NuA4 histone acetyltransferase complex
View computational annotations
Molecular Function
- Manually Curated
- enables histone acetyltransferase activity (IMP, IDA)
- enables histone crotonyltransferase activity (IDA)
- enables histone H4 acetyltransferase activity (IDA)
- enables peptide N-acetyltransferase activity (IMP)
- enables transcription coregulator activity (IDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in DNA repair (IMP, IDA, IGI)
- involved in DNA-templated transcription elongation (IDA, IMP)
- involved in positive regulation of macroautophagy (IMP)
- involved in positive regulation of transcription elongation by RNA polymerase II (IGI, IMP)
- involved in rDNA heterochromatin formation (IMP, IGI)
- involved in regulation of cell cycle (IMP)
- involved in regulation of transcription by RNA polymerase II (IMP)
Cellular Component
- Manually Curated
- located in chromatin (IDA)
- part of NuA4 histone acetyltransferase complex (IDA)
- part of piccolo histone acetyltransferase complex (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; conditional mutants exhibit heat sensitivity; in a large-scale study, depletion confers an Opi- phenotype, overproduction and excretion of inositol in the absence of inositol and choline
Classical Genetics
- conditional
- autophagy: decreased
- cell cycle progression through the G1/S phase transition: delayed
- chemical compound accumulation: increased
- heat sensitivity: increased
- nuclear export: decreased
- protein/peptide accumulation: decreased
- protein/peptide distribution: abnormal
- protein/peptide modification: absent
- protein/peptide modification: decreased
- resistance to chemicals: decreased
- RNA accumulation: decreased
- RNA accumulation: delayed
- RNA localization: abnormal
- silencing: decreased
- overexpression
- reduction of function
- unspecified
- null
- reduction of function
- overexpression
- repressible
- conditional
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Esa1p interacts physically with proteins involved in transcription; ESA1 interacts genetically with genes involved in transcription
1991 total interactions for 944 unique genes
Physical Interactions
- Affinity Capture-MS: 195
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 48
- Biochemical Activity: 126
- Co-crystal Structure: 1
- Co-fractionation: 1
- Co-localization: 2
- Co-purification: 28
- Reconstituted Complex: 8
- Two-hybrid: 3
Genetic Interactions
- Dosage Rescue: 12
- Negative Genetic: 407
- Phenotypic Enhancement: 8
- Phenotypic Suppression: 29
- Positive Genetic: 78
- Synthetic Growth Defect: 681
- Synthetic Lethality: 312
- Synthetic Rescue: 47
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- ESA1 promoter is bound by Xbp1p in response to heat; ESA1 transcription is regulated by Sfp1p
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-08-21
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.