FUS2 / YMR232W Overview
- Standard Name
- FUS2 1 2
- Systematic Name
- YMR232W
- SGD ID
- SGD:S000004845
- Feature Type
- ORF , Verified
- Description
- Cell fusion regulator; cytoplasmic protein localized to shmoo tip; required for alignment of parental nuclei before nuclear fusion during mating; contains a Dbl-homology domain; binds specifically with activated Cdc42p 2 3 4
- Name Description
- cell FUSion 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Fus2p is 677 amino acids long, low in abundance; contains a Dbl homology (DH) domain necessary for cell fusion; phosphorylated on 10 residues
- Length (a.a.)
- 677
- Mol. Weight (Da)
- 79053.0
- Isoelectric Point
- 9.25
- Median Abundance (molecules/cell)
- 3183 +/- 2896
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all FUS2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein involved in regulating the termination of mating projection growth as well as karyogamy during mating; localizes to nucleus and mating projection tip
View computational annotations
Molecular Function
- Manually Curated
- enables molecular function unknown (ND)
Biological Process
- Manually Curated
- involved in conjugation with cellular fusion (IMP)
- involved in cytogamy (IGI, IMP)
- involved in karyogamy involved in conjugation with cellular fusion (IMP)
Cellular Component
- Manually Curated
- located in cytoplasm (IDA)
- located in mating projection tip (IDA)
- located in mitochondrion (HDA)
- located in nucleus (HDA, IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null exhibits defective nuclear migration, vesicle accumulation, cell wall degradation and membrane fusion during cellular fusion, increased staining along the entire surface of the shmoo projection, extreme sensitivity to low levels of α-synuclein, deficient fast cell death, spontaneous direct-repeat hyper-recombination, decreased mating efficiency, longer mating projections, defective pheromone-induced relocalization of Fus2p to shmoo tip, decreased low-affinity Ca2+ influx; heterozygous null is sensitive to starvation and is haploinsufficient, which results in chromosome instability
Classical Genetics
- null
- cell fusion: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- mating efficiency: decreased
- mating projection morphology: abnormal
- necrotic cell death: decreased
- nuclear fusion during mating: decreased
- protein/peptide distribution: abnormal
- RNA accumulation: decreased
- reduction of function
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Fus2p interacts physically with proteins involved in cytoskeleton organization; FUS2 interacts genetically with genes involved in regulation of organelle organization
144 total interactions for 91 unique genes
Physical Interactions
- Affinity Capture-MS: 9
- Affinity Capture-RNA: 2
- Affinity Capture-Western: 5
- Biochemical Activity: 1
- Co-localization: 3
- Reconstituted Complex: 1
- Two-hybrid: 6
Genetic Interactions
- Dosage Rescue: 12
- Negative Genetic: 85
- Phenotypic Enhancement: 11
- Phenotypic Suppression: 5
- Positive Genetic: 3
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 7
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-06-24
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.