GTR1 / YML121W Overview
- Standard Name
- GTR1 1
- Systematic Name
- YML121W
- SGD ID
- SGD:S000004590
- Feature Type
- ORF , Verified
- Description
- Subunit of TORC1-stimulating GTPase and EGO/GSE complex; subunit of Gtr1-Gtr2 GTPase that activates TORC1 in response to amino acid stimulation; subunit of EGO/GSE vacuolar membrane complex that regulates exit from rapamycin-induced growth arrest and sorting of Gap1p; involved in phosphate transport, telomeric chromatin silencing and oxidative stress response; activated by Iml1p (GAP) subunit of SEACIT complex; targeted to vacuole via AP-3 pathway; similar to human RagA and RagB 1 2 3 4 5 6 7 8 9 10 11
- Name Description
- GTp binding protein Resemblance 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 310
- Mol. Weight (Da)
- 35850.9
- Isoelectric Point
- 8.47
- Median Abundance (molecules/cell)
- 2620 +/- 1077
- Half-life (hr)
- 11.0
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all GTR1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- GTPase that is subunit of the Gtr1-Gtr2 GTPase complex and the EGO complex; involved in TOR1 localization to the vacuolar membrane, positive regulation of TORC1 signaling, negative regulation of autophagy during nitrogen starvation, chromatin silencing at telomeres, and transcription from RNA polymerase I and III promoters; also involved in phosphate ion transport; localizes to the nucleus, cytoplasm, vacuole membrane, and late endosome membrane
View computational annotations
Molecular Function
- Manually Curated
- enables GTPase activity (IDA)
Biological Process
- Manually Curated
- involved in cellular response to amino acid stimulus (IMP)
- involved in cellular response to nitrogen starvation (IMP, IGI)
- acts upstream of or within cellular response to oxidative stress (IMP)
- involved in negative regulation of autophagy (IGI, IMP)
- involved in phosphate ion transport (IMP)
- involved in positive regulation of TORC1 signaling (IGI, IDA, IMP)
- involved in protein localization to vacuolar membrane (IMP)
- involved in subtelomeric heterochromatin formation (IMP)
- involved in transcription by RNA polymerase I (IMP, IPI)
- involved in transcription by RNA polymerase III (IPI, IMP)
Cellular Component
- Manually Curated
- located in cytoplasm (IDA)
- located in fungal-type vacuole membrane (HDA, IDA)
- part of Gtr1-Gtr2 GTPase complex (IPI)
- located in late endosome membrane (IDA)
- located in nucleus (IDA)
- part of Ragulator complex (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; mutants show defects in vacuolar delivery of reporter proteins, and are sensitive to temperatures, cell wall-affecting drugs, sirolimus (rapamycin); also show decreased telomere length and reduced competitive fitness
Classical Genetics
- unspecified
- null
- cell death: decreased rate
- chemical compound accumulation: increased
- cold sensitivity: increased
- growth in exponential phase: decreased rate
- heat sensitivity: increased
- hydrostatic pressure resistance: decreased
- metal resistance: decreased
- nutrient uptake: decreased
- nutrient uptake: decreased rate
- oxidative stress resistance: decreased
- protein/peptide distribution: abnormal
- protein/peptide modification: absent
- protein/peptide modification: decreased
- protein/peptide modification: delayed
- protein/peptide modification: increased
- replicative lifespan: abnormal
- replicative lifespan: increased
- resistance to chemicals: decreased
- overexpression
- reduction of function
- activation
- repressible
- null
- biofilm formation: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- cold sensitivity: increased
- competitive fitness: decreased
- filamentous growth: decreased
- haploinsufficient
- heat sensitivity: decreased
- heat sensitivity: increased
- hydrostatic pressure resistance: decreased
- innate thermotolerance: increased
- invasive growth: absent
- killer toxin resistance: increased
- metal resistance: decreased
- oxidative stress resistance: decreased
- oxidative stress resistance: increased
- protein activity: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- silencing: decreased
- stress resistance: increased
- telomere length: decreased
- vegetative growth: decreased rate
- viable
- unspecified
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
680 total interactions for 441 unique genes
Physical Interactions
- Affinity Capture-MS: 25
- Affinity Capture-RNA: 7
- Affinity Capture-Western: 27
- Biochemical Activity: 1
- Co-crystal Structure: 2
- Co-fractionation: 2
- Co-localization: 4
- Co-purification: 1
- PCA: 19
- Reconstituted Complex: 18
- Two-hybrid: 17
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 1
- Dosage Rescue: 10
- Negative Genetic: 337
- Phenotypic Enhancement: 17
- Phenotypic Suppression: 70
- Positive Genetic: 79
- Synthetic Growth Defect: 10
- Synthetic Haploinsufficiency: 1
- Synthetic Lethality: 5
- Synthetic Rescue: 26
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 8
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.