MATALPHA2 / YCR039C Overview
- Standard Name
- MATALPHA2 1
- Systematic Name
- YCR039C
- SGD ID
- SGD:S000000635
- Aliases
- ALPHA2
- Feature Type
- ORF , Verified
- Description
- Homeobox-domain protein; with Mcm1p, represses a-specific genes in haploids; acts with A1p to repress transcription of haploid-specific genes in diploids; one of two genes encoded by the MATalpha mating type cassette 2 3
- Name Description
- MATing type protein ALPHA
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Homeobox-domain protein
- Length (a.a.)
- 210
- Mol. Weight (Da)
- 24291.4
- Isoelectric Point
- 10.14
- Median Abundance (molecules/cell)
- 333 +/- 158
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all MATALPHA2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence-specific DNA-binding transcription repressor involved in the negative regulation of mating-type specific transcription by RNA polymerase II
View computational annotations
Molecular Function
- Manually Curated
- enables DNA binding, bending (IDA)
- enables DNA-binding transcription repressor activity, RNA polymerase II-specific (IDA)
- enables sequence-specific DNA binding (IDA)
Biological Process
- Manually Curated
- involved in negative regulation of transcription by RNA polymerase II (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutants show decreased mating and sporulation efficiency; overexpression interferes with cellular morphology and budding pattern, and can be lethal
Classical Genetics
- overexpression
- null
- reduction of function
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
39 total interactions for 22 unique genes
Physical Interactions
- Affinity Capture-Western: 1
- Biochemical Activity: 4
- Co-crystal Structure: 4
- Co-localization: 1
- Reconstituted Complex: 12
- Two-hybrid: 1
Genetic Interactions
- Dosage Lethality: 4
- Dosage Rescue: 2
- Negative Genetic: 8
- Phenotypic Enhancement: 1
- Positive Genetic: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- MATALPHA2 encodes a helix-turn-helix class transcription factor of the homeodomain family. MATalpha2p cooperates with other transcription factors to regulate the transcription of mating type-specific genes. As a heterodimer with Mcm1p, it represses transcription of mating type (MAT) a-specific genes in MAT alpha cells and in diploid cells. MATalpha2p-Mcm1p recruits the general repressor complex Tup1p-Cyc8p to promoters. The targets of MATalpha2p-Mcm1p include the a-factor genes MFA1 and MFA2, and the STE3 gene encoding the cell surface receptor for alpha-factor. Binding to the homeodomain protein MATa1p, which on its own cannot bind DNA, alters the DNA binding specificity of MATalpha2p to repress both MAT alpha-specific genes and haploid-specific genes in diploid cells. Examples of MATa1p-MATalpha2p targets include MATalpha1, a transcriptional activator of alpha-specific genes; RME1, a transcriptional repressor of meiotic genes; and HO, encoding the site-specific nuclease required for mating type switching. Recently, it has also been shown that MATalpha2p interacts with Fyv5p and the two factors bind cooperatively to the promoters of haploid-specific genes to repress transcription in diploids. Both the MATalpha2p-Mcm1p and MATa1p-MATalpha2p complexes cause DNA bending as they bind DNA. MATalpha2p is degraded via the ubiquitin-mediated proteasomal degradation pathway. Alone, it is short-lived, but its degradation is regulated by its binding partners: binding to Tup1p-Cyc8p or to MATa1p masks degradation signals and stabilizes MATalpha2p.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2004-11-05
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.