NTG1 / YAL015C Overview
- Standard Name
- NTG1 1
- Systematic Name
- YAL015C
- SGD ID
- SGD:S000000013
- Aliases
- FUN33 8 , SCR1 , ogg2 9 10
- Feature Type
- ORF , Verified
- Description
- DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; required for maintaining mitochondrial genome integrity; NTG1 has a paralog, NTG2, that arose from the whole genome duplication 2 3 4 5 6 7
- Name Description
- eNdonuclease Three-like Glycosylase 1
- Paralog
- NTG2 6
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- NTG1 is located on the left arm of chromosome I between TPD3 heterotrimeric PP2A complex subunit and SYN8 endosomal SNARE; coding sequence is 1200 nucleotides long with 6 nonsynonymous SNPs, 13 synonymous SNPs; NTG1 has paralog NTG2 from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 399
- Mol. Weight (Da)
- 45579.4
- Isoelectric Point
- 8.18
- Median Abundance (molecules/cell)
- 1154 +/- 404
- Half-life (hr)
- 7.8
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all NTG1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Oxidized nucleobase lesion DNA N-glycosylase and endonuclease involved in base-excision repair, upregulation of mitochondrial DNA replication, and oxidative stress response; localizes to nucleus and mitochondrion
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in base-excision repair (IDA)
- involved in base-excision repair, AP site formation (IDA)
- involved in cellular response to oxidative stress (IMP)
- involved in DNA repair (IDA)
- involved in positive regulation of mitochondrial DNA replication (IMP)
Cellular Component
- Manually Curated
- located in mitochondrion (HDA, IDA)
- located in nucleus (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant shows increased mutation rate and fails to increase mitochondrial genome copy number after oxidative stress;
Classical Genetics
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The ntg1 null mutant is viable; the null mutant of paralog ntg2 is viable; the ntg1 ntg2 double mutant is inviable or displays a growth defect; an ntg1 ntg2 apn1 mutant is viable but has an increased mutation rate and increased sensitivity to DNA damaging agents.
142 total interactions for 114 unique genes
Physical Interactions
- Affinity Capture-MS: 19
- Affinity Capture-RNA: 4
- Biochemical Activity: 1
- Co-purification: 1
Genetic Interactions
- Dosage Rescue: 1
- Negative Genetic: 56
- Phenotypic Enhancement: 16
- Phenotypic Suppression: 12
- Positive Genetic: 8
- Synthetic Growth Defect: 13
- Synthetic Lethality: 7
- Synthetic Rescue: 4
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 7
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.