PBS2 / YJL128C Overview
- Standard Name
- PBS2 1
- Systematic Name
- YJL128C
- SGD ID
- SGD:S000003664
- Aliases
- HOG4 5 , SFS4 , SSK4 23 24
- Feature Type
- ORF , Verified
- Description
- MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition 2 3 4
- Name Description
- Polymyxin B Sensitivity 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 668
- Mol. Weight (Da)
- 72731.9
- Isoelectric Point
- 9.44
- Median Abundance (molecules/cell)
- 3777 +/- 580
- Half-life (hr)
- 9.7
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all PBS2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- MAP kinase kinase and scaffold protein involved in the osmosensory signaling pathway and hyperosmotic response; involved in activation of MAPK activity and MAPK nuclear import during osmolarity sensing; has a role in actin filament organization; localizes to the cytoplasm, bud neck and tip, and cytoplasmic stress granule
View computational annotations
Molecular Function
- Manually Curated
- enables MAP kinase kinase activity (IMP, ISS)
- enables MAP-kinase scaffold activity (IPI)
- enables protein binding (IPI)
- enables protein kinase activity (HDA)
Biological Process
- Manually Curated
- involved in actin filament organization (IMP)
- involved in cellular hyperosmotic response (IGI)
- involved in hyperosmotic response (IMP)
- involved in osmosensory signaling pathway (IMP)
- involved in osmosensory signaling pathway via Sho1 osmosensor (IPI)
- involved in p38MAPK cascade (IGI, IMP)
- involved in protein import into nucleus (IMP)
Cellular Component
- Manually Curated
- located in cellular bud neck (IDA)
- located in cellular bud tip (IDA)
- is active in cytoplasm (IDA)
- located in cytoplasmic stress granule (HDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant displays phenotypes associated with a defective cell wall, including sensitivity to killer toxin, reducing agents, SDS, and zymolyase, and resistance to Calcofluor White; null mutant accumulates elevated levels of cell wall (1->6)-beta-D-glucan and has elongated cell shape; in large-scale studies, null mutant has reduced competitive fitness, reduced resistance to hyperosmotic stress, decreased endocytosis and desiccation resistance, and altered resistance to various chemicals
Classical Genetics
- null
- acid pH resistance: decreased
- budding pattern: abnormal
- cell shape: abnormal
- chemical compound accumulation: abnormal
- chemical compound accumulation: increased
- cytokinesis: absent
- invasive growth: increased
- killer toxin resistance: decreased
- mating response: increased
- metal resistance: decreased
- mitophagy: decreased
- osmotic stress resistance: decreased
- oxidative stress resistance: decreased
- protein/peptide distribution: abnormal
- protein/peptide modification: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- stress resistance: decreased
- toxin resistance: increased
- overexpression
- conditional
- unspecified
- null
- acid pH resistance: decreased
- auxotrophy
- chemical compound accumulation: abnormal
- chemical compound accumulation: increased
- chromosome/plasmid maintenance: decreased
- competitive fitness: decreased
- competitive fitness: increased
- desiccation resistance: decreased
- endocytosis: decreased
- haploinsufficient
- hyperosmotic stress resistance: decreased
- innate thermotolerance: decreased
- killer toxin resistance: decreased
- mating response: increased
- metal resistance: decreased
- oxidative stress resistance: decreased
- oxidative stress resistance: increased
- protein/peptide accumulation: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- stress resistance: decreased
- transposable element transposition: increased
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
944 total interactions for 521 unique genes
Physical Interactions
- Affinity Capture-MS: 37
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 14
- Biochemical Activity: 14
- Co-purification: 1
- PCA: 8
- Protein-peptide: 2
- Proximity Label-MS: 1
- Reconstituted Complex: 9
- Two-hybrid: 8
Genetic Interactions
- Dosage Growth Defect: 2
- Dosage Lethality: 1
- Dosage Rescue: 29
- Negative Genetic: 634
- Phenotypic Enhancement: 9
- Phenotypic Suppression: 7
- Positive Genetic: 118
- Synthetic Growth Defect: 12
- Synthetic Haploinsufficiency: 2
- Synthetic Lethality: 4
- Synthetic Rescue: 27
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 13
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2007-01-25
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.