PDR5 / YOR153W Overview


Standard Name
PDR5 1
Systematic Name
YOR153W
SGD ID
SGD:S000005679
Aliases
LEM1 30 , YDR1 31 , STS1 4
Feature Type
ORF , Verified
Description
Plasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter actively regulated by Pdr1p; also involved in steroid transport, cation resistance, and cellular detoxification during exponential growth; PDR5 has a paralog, PDR15, that arose from the whole genome duplication 2 3 4 5 6 7 8 9 10 11
Name Description
Pleiotropic Drug Resistance 1
Paralog
PDR15 11
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
PDR5 is located on the right arm of chromosome XV between ATG40 autophagy receptor and SLP1 ER membrane protein; coding sequence is 4536 nucleotides long with 41 SNPs, 12 of which cause an amino acid polymorphism; PDR5 has a paralog, PDR15, that arose from the whole genome duplication
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Pdr5p is 1511 amino acids long, shorter-lived, moderate in abundance; contains 2 ATP-binding domains; N-glycosylated on N57 and N734, succinylated on K232, ubiquitinylated on 5 lysines, phosphorylated on 34 residues
Length (a.a.)
1511
Mol. Weight (Da)
170444.9
Isoelectric Point
7.87
Median Abundance (molecules/cell)
10371 +/- 4608
Half-life (hr)
8.2

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all PDR5 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Plasma membrane ATP-binding cassette (ABC) transporter involved in resistance to multiple drugs; facilitates drug removal from the cell

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null and point mutations confer resistance or sensitivity to various drugs; in large-scale studies, null mutation confers a filamentous growth defect, increased competitive fitness in minimal medium, and altered resistance to a wide variety of drugs
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Pdr5p interacts physically with proteins involved in transmembrane transport, PDR5 interacts genetically with genes involved in transcription; the pdr5 null mutant is viable, the null mutant of paralog pdr15 is viable, the pdr5 pdr15 double mutant displays a phenotypic enhancement

227 total interactions for 163 unique genes

Physical Interactions

  • Affinity Capture-MS: 57
  • Affinity Capture-RNA: 10
  • Affinity Capture-Western: 4
  • Biochemical Activity: 2
  • Co-fractionation: 10
  • PCA: 54
  • Two-hybrid: 13

Genetic Interactions

  • Dosage Rescue: 6
  • Negative Genetic: 22
  • Phenotypic Enhancement: 16
  • Phenotypic Suppression: 7
  • Synthetic Growth Defect: 21
  • Synthetic Rescue: 5
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
PDR5 transcription is upregulated by Pdr1p, Pdr3p, and SWI/SNF in response to xenobiotics; PDR5 transcription is also upregulated by Rtt106p and Ume6p
Regulators
20
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2007-11-06

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
249
Additional
254
Reviews
75

Resources