PMR1 / YGL167C Overview
- Standard Name
- PMR1 1
- Systematic Name
- YGL167C
- SGD ID
- SGD:S000003135
- Aliases
- SSC1 1 , BSD1 18 , LDB1 19
- Feature Type
- ORF , Verified
- Description
- High affinity Ca2+/Mn2+ P-type ATPase; required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting, processing; D53A mutant (Mn2+ transporting) is rapamycin sensitive, Q783A mutant (Ca2+ transporting) is rapamycin resistant; Mn2+ transport into Golgi lumen required for rapamycin sensitivity; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease; human ATP2C1 can complement yeast null mutant 2 3 4 5 6 7 8
- Name Description
- Plasma Membrane ATPase Related 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 950
- Mol. Weight (Da)
- 104554.8
- Isoelectric Point
- 5.36
- Median Abundance (molecules/cell)
- 7303 +/- 3248
- Half-life (hr)
- 10.2
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all PMR1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Calcium and manganese-transporting ATPase involved in calcium ion transport and homeostasis, manganese ion transport, macroautophagy, and exocytosis; localizes to the Golgi membrane
View computational annotations
Molecular Function
- Manually Curated
- enables calcium ion binding (IDA)
- enables P-type calcium transporter activity (IDA, IMP)
- enables P-type manganese transporter activity (IDA)
Biological Process
- Manually Curated
- involved in calcium ion transport (IMP, IDA)
- involved in intracellular calcium ion homeostasis (IGI)
- involved in intracellular manganese ion homeostasis (IGI, IMP)
- involved in macroautophagy (IMP)
- involved in manganese ion transport (IDA)
Cellular Component
- Manually Curated
- located in Golgi membrane (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant requires inositol for growth and is sensitive to calcium and magnesium ions, alkaline pH, and reducing agents; vacuoles of null mutant have multiple lobes or are fragmented; null mutant has shorter replicative lifespan and reduced mating efficiency, and accumulates excess triglycerides, sterol esters, and calcium ions; in large-scale studies, null mutant displays reduced competitive fitness, desiccation resistance, heat resistance, and freeze-thaw resistance, altered resistance to various chemicals, and poor utilization of several nitrogen sources
Classical Genetics
- null
- alkaline pH resistance: decreased
- autophagy: decreased
- autophagy: increased
- auxotrophy
- cell cycle progression in S phase: increased duration
- chemical compound accumulation: increased
- mating efficiency: decreased
- meiosis: abnormal
- metal resistance: decreased
- metal resistance: increased
- mitophagy: decreased
- oxidative stress resistance: decreased
- pheromone production: decreased
- protein activity: decreased
- protein activity: increased
- protein transport: decreased
- protein/peptide accumulation: increased
- protein/peptide modification: abnormal
- protein/peptide modification: decreased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- respiratory growth: increased
- small molecule transport: increased
- telomere length: decreased
- toxin resistance: decreased
- vacuolar morphology: abnormal
- vegetative growth: decreased
- vegetative growth: increased
- overexpression
- reduction of function
- null
- alkaline pH resistance: decreased
- auxotrophy
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chemical compound excretion: increased
- colony appearance: abnormal
- competitive fitness: decreased
- desiccation resistance: decreased
- endocytosis: decreased
- endoplasmic reticulum morphology: abnormal
- freeze-thaw resistance: decreased
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- killer toxin resistance: increased
- liquid culture appearance: abnormal
- metal resistance: decreased
- metal resistance: increased
- mitophagy: absent
- oxidative stress resistance: decreased
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- respiratory growth: decreased
- respiratory growth: decreased rate
- starvation resistance: decreased
- toxin resistance: decreased
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- vegetative growth: decreased
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast PMR1 is homologous to human ATP2C1, and has been used to study Hailey-Hailey disease
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
1613 total interactions for 873 unique genes
Physical Interactions
- Affinity Capture-MS: 85
- Affinity Capture-RNA: 6
- Co-localization: 3
- Co-purification: 1
- PCA: 2
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 14
- Dosage Rescue: 17
- Negative Genetic: 1135
- Phenotypic Enhancement: 18
- Phenotypic Suppression: 81
- Positive Genetic: 139
- Synthetic Growth Defect: 74
- Synthetic Lethality: 18
- Synthetic Rescue: 19
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 2
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2006-11-21
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.