POL32 / YJR043C Overview
- Standard Name
- POL32 1
- Systematic Name
- YJR043C
- SGD ID
- SGD:S000003804
- Aliases
- REV5 11
- Feature Type
- ORF , Verified
- Description
- Third subunit of DNA polymerase delta; involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; parental H3-H4 histone chaperone that mediates transfer of parental histones to the lagging strand via Pol1p during DNA replication; forms a complex with Rev3p, Rev7p and Pol31p; interacts with Hys2p, PCNA (Pol30p), and Pol1p 2 3 4 5
- Name Description
- POLymerase 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Pol32p is 350 amino acids long, shorter-lived, and low in abundance; sumoylated on K283, phosphorylated on 17 residues
- Length (a.a.)
- 350
- Mol. Weight (Da)
- 40314.2
- Isoelectric Point
- 8.63
- Median Abundance (molecules/cell)
- 1363 +/- 530
- Half-life (hr)
- 5.3
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all POL32 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Subunit of DNA polymerase delta; contributes to DNA polymerase activity and single-stranded DNA 3'-5' exodeoxyribonuclease activity; involved in RNA-dependent DNA biosynthesis, leading and lagging strand elongation, Okazaki initiator RNA removal, DNA amplification, and DNA repair
View computational annotations
Molecular Function
- Manually Curated
- enables DNA-directed DNA polymerase activity (IDA, TAS)
- enables H3-H4 histone complex chaperone activity (IMP, IDA)
- contributes to single-stranded DNA 3'-5' DNA exonuclease activity (TAS)
Biological Process
- Manually Curated
- involved in base-excision repair (TAS)
- involved in DNA amplification (IMP)
- involved in DNA replication, removal of RNA primer (IDA)
- involved in double-strand break repair via break-induced replication (IMP)
- involved in error-prone translesion synthesis (IMP)
- involved in lagging strand elongation (TAS)
- involved in leading strand elongation (TAS)
- involved in nucleotide-excision repair (TAS)
- involved in RNA-templated DNA biosynthetic process (IDA)
Cellular Component
- Manually Curated
- part of delta DNA polymerase complex (TAS)
- located in nucleus (HDA)
- part of zeta DNA polymerase complex (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant displays chromosome instability and sensitivity to cold, HU, and selenite; in large-scale studies, null mutant displays reduced competitive fitness in a variety of media, is sensitive to heat and desiccation, has elongated telomeres, and shows altered resistance to a variety of chemicals
Classical Genetics
- null
- overexpression
- null
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chromosome/plasmid maintenance: abnormal
- competitive fitness: decreased
- desiccation resistance: decreased
- fermentative growth: decreased rate
- heat sensitivity: decreased
- heat sensitivity: increased
- metal resistance: decreased
- mitotic recombination: increased
- mutation frequency: increased
- nuclear morphology: abnormal
- oxidative stress resistance: decreased
- protein/peptide accumulation: increased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: increased
- telomere length: increased
- toxin resistance: decreased
- vacuolar morphology: abnormal
- viable
- overexpression
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Pol32p interacts physically with proteins involved in DNA repair; POL32 interacts genetically with genes involved in DNA repair
1246 total interactions for 461 unique genes
Physical Interactions
- Affinity Capture-MS: 11
- Affinity Capture-RNA: 8
- Affinity Capture-Western: 17
- Biochemical Activity: 1
- Co-crystal Structure: 3
- Co-purification: 5
- FRET: 1
- Protein-peptide: 1
- Reconstituted Complex: 22
- Two-hybrid: 15
Genetic Interactions
- Dosage Growth Defect: 3
- Dosage Lethality: 7
- Dosage Rescue: 1
- Negative Genetic: 684
- Phenotypic Enhancement: 28
- Phenotypic Suppression: 29
- Positive Genetic: 40
- Synthetic Growth Defect: 237
- Synthetic Lethality: 90
- Synthetic Rescue: 43
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 4
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2005-09-15
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.