PZF1 / YPR186C Overview
- Standard Name
- PZF1 1
- Systematic Name
- YPR186C
- SGD ID
- SGD:S000006390
- Aliases
- TFC2 3 , TFIIIA
- Feature Type
- ORF , Verified
- Description
- Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA 1 2 3 4
- Name Description
- Putative Zinc Finger 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 429
- Mol. Weight (Da)
- 50040.7
- Isoelectric Point
- 8.67
- Median Abundance (molecules/cell)
- 1455 +/- 496
- Half-life (hr)
- 7.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all PZF1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- RNA polymerase III transcription factor essential for sequence-specific DNA binding within RNA polymerase III type 1 promoters; involved in transcription of 5S class rRNA by RNA polymerase III; localized to the nucleus
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in 5S class rRNA transcription by RNA polymerase III (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; overexpression increases resistance to sirolimus (rapamycin) under competitive growth conditions; in systematic studies, overexpression increases filamentous growth in strain Σ1278b
Classical Genetics
- null
- null
- overexpression
- reduction of function
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
99 total interactions for 99 unique genes
Physical Interactions
- Affinity Capture-MS: 2
- Affinity Capture-RNA: 3
Genetic Interactions
- Negative Genetic: 88
- Positive Genetic: 3
- Synthetic Lethality: 3
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- PZF1 encodes an RNA polymerase III transcription factor, TFIIIA, that is specific for the 5S rRNA genes. Pzf1p binds to the Internal Control Region (ICR) of 5S rRNA genes and mediates promoter recognition by RNA polymerase III. Pzf1p bound to DNA first recruits TFIIIC, a six-subunit complex. The TFIIIA-TFIIIC-DNA complex then recruits TFIIIB, a three-subunit complex, and finally RNA polymerase III. Pzf1p has nine Cys2His2 zinc fingers, some of which bind DNA and some of which contribute to interaction with TFIIIC.
- Regulators
- 3
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.