RAD54 / YGL163C Overview
- Standard Name
- RAD54 1
- Systematic Name
- YGL163C
- SGD ID
- SGD:S000003131
- Aliases
- XRS1
- Feature Type
- ORF , Verified
- Description
- DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; drives ATP-hydrolysis-dependent sequence alignment during homologous recombination; involved in repair of dsDNA breaks during both mitosis and meiosis; promotes strand invasion, catalyzes nucleosome remodeling, and removes Rad51p from dsDNA; member of the SWI/SNF family of DNA translocases; forms nuclear foci upon DNA replication stress 2 3 4 5 6 7 8 9 10
- Name Description
- RADiation sensitive 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 898
- Mol. Weight (Da)
- 101778.9
- Isoelectric Point
- 9.41
- Median Abundance (molecules/cell)
- 782 +/- 582
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all RAD54 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- DNA-dependent ATPase and translocase involved in chromatin remodeling, induction of DNA geometric changes and heteroduplex formation; enhances the activity of the structure-specific Mms4p-Mus81p endonuclease; also involved in telomerase-independent telomere maintenance via recombination
View computational annotations
Molecular Function
- Manually Curated
- enables DNA translocase activity (IDA, IMP)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in chromatin remodeling (IDA)
- involved in DNA geometric change (IDA)
- involved in heteroduplex formation (IDA)
- involved in telomere maintenance via recombination (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant is sensitive to DNA damaging agents, DNA synthesis and topoisomerase inhibitors; null mutant is sensitive to X-rays, gamma rays and UV radiation; null displays increased chromosome instability, increased loss-of-heterozygosity in diploids, increased mutation rate and elevated Ty element transposition; null mutant has abnormally elongated buds and decreased duration of the G1 phase; null mutant is temperature sensitive, and displays decreased competitive fitness on rich and synthetic media; null mutant is unable to switch mating types, has increased stationary phase thermotolerance, and decreased oxidative stress resistance; diploid null displays increased sporulation efficiency and haploid has decreased spore germination; null contains large-budded cells with nuclear extensions at the nuclear envelope, adjacent to the nucleolus
Classical Genetics
- null
- apoptosis: decreased
- cell cycle progression in G1 phase: decreased duration
- cellular morphology: abnormal
- chromosome/plasmid maintenance: abnormal
- colony sectoring: increased
- growth in exponential phase: decreased rate
- mating type switching: absent
- mutation frequency: increased rate
- resistance to chemicals: decreased
- reduction of function
- conditional
- null
- bud morphology: abnormal
- chemical compound accumulation: abnormal
- chemical compound accumulation: increased
- competitive fitness: decreased
- endoplasmic reticulum morphology: abnormal
- gamma ray resistance: decreased
- haploproficient
- heat sensitivity: increased
- innate thermotolerance: increased
- mitotic recombination: decreased
- mutation frequency: increased
- nuclear morphology: abnormal
- oxidative stress resistance: decreased
- protein/peptide accumulation: increased
- resistance to chemicals: decreased
- resistance to enzymatic treatment: decreased
- sporulation efficiency: increased
- stress resistance: increased
- toxin resistance: decreased
- transposable element transposition: increased
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- UV resistance: decreased
- vacuolar morphology: abnormal
- viable
- X ray resistance: decreased
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
656 total interactions for 310 unique genes
Physical Interactions
- Affinity Capture-MS: 4
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 7
- Biochemical Activity: 2
- Co-localization: 1
- Reconstituted Complex: 23
- Two-hybrid: 11
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 2
- Dosage Rescue: 5
- Negative Genetic: 277
- Phenotypic Enhancement: 29
- Phenotypic Suppression: 13
- Positive Genetic: 65
- Synthetic Growth Defect: 142
- Synthetic Lethality: 45
- Synthetic Rescue: 25
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 9
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2006-05-03
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.