BIK1 / YCL029C Overview
- Standard Name
- BIK1 1
- Systematic Name
- YCL029C
- SGD ID
- SGD:S000000534
- Aliases
- ARM5 , PAC14 6
- Feature Type
- ORF , Verified
- Description
- Microtubule-associated protein; component of the interface between microtubules and the kinetochore; involved in sister chromatid separation and mitotic spindle elongation; involved in karyogamy during mating; stabilizes Kip2p at microtubule plus ends and recruits Kip2p to mitotic SPBs; essential in polyploid cells but not in haploid or diploid cells; promotes plus-end directed movement of Dyn1p; ortholog of mammalian CLIP-170 2 3 4 5
- Name Description
- BIlateral Karyogamy defect 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 440
- Mol. Weight (Da)
- 51078.6
- Isoelectric Point
- 5.76
- Median Abundance (molecules/cell)
- 1830 +/- 1007
- Half-life (hr)
- 11.6
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all BIK1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Microtubule-binding protein involved in 2-micrometer plasmid partitioning, nuclear migration and karyogamy during mating, mitotic spindle elongation, and negative regulation of microtubule polymerization; has protein homodimerization activity; localizes to kinetochores, microtubule plus-ends, the mating projection tip, the cell cortex, and the spindle and spindle pole body
View computational annotations
Molecular Function
- Manually Curated
- enables kinesin binding (IDA)
- enables microtubule binding (IDA)
Biological Process
- Manually Curated
- involved in 2-micrometer plasmid partitioning (IGI, IMP)
- involved in karyogamy involved in conjugation with cellular fusion (IMP)
- acts upstream of or within microtubule nucleation (IMP)
- involved in mitotic spindle elongation (IGI)
- involved in negative regulation of microtubule polymerization (IDA)
- involved in nuclear migration involved in conjugation with cellular fusion (IMP)
- acts upstream of or within protein localization to mitotic spindle pole body (IMP)
- involved in sister chromatid segregation (IMP)
Cellular Component
- Manually Curated
- located in cell cortex (IDA)
- is active in kinetochore (IDA)
- located in mating projection tip (IDA)
- located in microtubule plus-end (IDA)
- located in spindle (IDA)
- located in spindle microtubule (IDA)
- located in spindle pole body (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant has both a decreased growth rate and competitive fitness and is both heat and cold sensitive; diploid null mutant displays a reduced number and length of cytoplasmic microtubules, short and broken anaphase spindles, and abnormally positioned nuclei resulting in anucleate and multinucleate cells; null mutant has a strong bilateral karyogamy defect; null mutant has an elevated rate of chromosome loss, is benomyl sensitive and displays an increased fraction of cells with nuclear extensions at the nuclear envelope; overexpression results in chromosomal instability, and a large-budded cell cycle arrest where cells contain a single nucleus located in or near the neck and abnormal cytoplasmic microtubules; heterozygous null mutant is haploproficient
Classical Genetics
- overexpression
- null
- reduction of function
- repressible
- null
- competitive fitness: decreased
- desiccation resistance: decreased
- endoplasmic reticulum morphology: abnormal
- fermentative growth: decreased rate
- haploproficient
- heat sensitivity: decreased
- heat sensitivity: increased
- innate thermotolerance: decreased
- nuclear morphology: abnormal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- stress resistance: decreased
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- vegetative growth: decreased
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
495 total interactions for 253 unique genes
Physical Interactions
- Affinity Capture-MS: 31
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 14
- Biochemical Activity: 6
- Co-crystal Structure: 1
- Co-fractionation: 1
- Co-localization: 6
- Co-purification: 1
- PCA: 7
- Protein-peptide: 1
- Proximity Label-MS: 2
- Reconstituted Complex: 13
- Two-hybrid: 63
Genetic Interactions
- Dosage Growth Defect: 2
- Negative Genetic: 228
- Phenotypic Enhancement: 6
- Phenotypic Suppression: 2
- Positive Genetic: 17
- Synthetic Growth Defect: 27
- Synthetic Lethality: 62
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 5
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.