SPF1 / YEL031W Overview
- Standard Name
- SPF1 1
- Systematic Name
- YEL031W
- SGD ID
- SGD:S000000757
- Aliases
- PIO1 7 , PER9 8 , COD1 2
- Feature Type
- ORF , Verified
- Description
- P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid and sterol composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1; null mutation is complemented by human Parkinson disease-related ATP13A1 1 2 3 4 5 6
- Name Description
- Sensitivity to Pichia Farinosa killer toxin 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 1215
- Mol. Weight (Da)
- 135259.6
- Isoelectric Point
- 6.56
- Median Abundance (molecules/cell)
- 15064 +/- 5429
- Half-life (hr)
- 11.7
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SPF1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- P-type ATPase involved in the homeostasis of calcium, manganese, and sterol; localizes to the endoplasmic reticulum membrane and the cis-Golgi network
View computational annotations
Molecular Function
- Manually Curated
- enables phosphatidylinositol-4-phosphate binding (IDA)
Biological Process
- Manually Curated
- involved in intracellular calcium ion homeostasis (IMP)
- involved in intracellular manganese ion homeostasis (IMP)
- involved in sterol homeostasis (IMP)
Cellular Component
- Manually Curated
- located in cis-Golgi network (IDA)
- located in endoplasmic reticulum (HDA)
- located in endoplasmic reticulum membrane (IDA)
- located in mitochondrion (HDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant has abnormal distribution of protein reporters throughout organelles and abnormal distribution of ergosterol; null mutant cells show increased aggregation and are hypersensitive to Calcofluor White and hygromycin B; null mutant is also sensitive to aureobasidin A, tunicamycin, terbinafine, nicotinamide; both null mutation and overexpression decrease growth rate
Classical Genetics
- null
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- growth in exponential phase: decreased rate
- killer toxin resistance: increased
- lipid particle morphology: abnormal
- liquid culture appearance: abnormal
- protein secretion: decreased
- protein/peptide accumulation: decreased
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- protein/peptide modification: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- transposable element transposition: decreased
- viable
- null
- anaerobic growth: decreased
- auxotrophy
- biofilm formation: increased
- chemical compound accumulation: abnormal
- chemical compound excretion: increased
- chitin deposition: normal
- chronological lifespan: decreased
- competitive fitness: decreased
- desiccation resistance: decreased
- endocytosis: decreased
- heat sensitivity: increased
- innate thermotolerance: decreased
- killer toxin resistance: increased
- peroxisomal morphology: abnormal
- protein/peptide distribution: abnormal
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- sporulation: decreased
- starvation resistance: decreased
- stress resistance: decreased
- toxin resistance: decreased
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
921 total interactions for 504 unique genes
Physical Interactions
- Affinity Capture-MS: 74
- Affinity Capture-RNA: 6
- Co-localization: 1
- PCA: 2
- Proximity Label-MS: 1
Genetic Interactions
- Negative Genetic: 653
- Phenotypic Enhancement: 2
- Phenotypic Suppression: 48
- Positive Genetic: 76
- Synthetic Growth Defect: 29
- Synthetic Lethality: 27
- Synthetic Rescue: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 4
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.