CIN8 / YEL061C Overview
- Standard Name
- CIN8 1
- Systematic Name
- YEL061C
- SGD ID
- SGD:S000000787
- Aliases
- KSL2 3 , SDS15 6
- Feature Type
- ORF , Verified
- Description
- Bipolar kinesin motor protein; switches from minus- to plus-end-directed motility in vitro depending on conditions; involved in mitotic spindle assembly and chromosome segregation 1 2 3 5
- Name Description
- Chromosome INstability 4
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Cin8p is 1000 amino acids long, extremely short-lived, extremely low in abundance; contains disordered regions at each terminus as well as 4 kinesin motor domains; phosphorylated on 11 residues
- Length (a.a.)
- 1000
- Mol. Weight (Da)
- 113316.4
- Isoelectric Point
- 8.28
- Median Abundance (molecules/cell)
- 763 +/- 682
- Half-life (hr)
- 1.3
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CIN8 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- ATP-dependent microtubule motor involved in microtubule depolymerization, mitotic spindle assembly and elongation, spindle pole body separation, and sister chromatid segregation; localizes to astral microtubules and condensed nuclear chromosome kinetochores
View computational annotations
Molecular Function
- Manually Curated
- enables microtubule motor activity (IGI)
- enables minus-end-directed microtubule motor activity (IDA, IMP)
- enables plus-end-directed microtubule motor activity (IDA)
Biological Process
- Manually Curated
- involved in initial mitotic spindle pole body separation (IGI, IMP)
- involved in meiotic sister chromatid segregation (IMP)
- involved in microtubule depolymerization (IMP)
- involved in mitotic sister chromatid segregation (IGI)
- involved in mitotic spindle assembly (IMP)
- involved in mitotic spindle elongation (IMP)
- involved in regulation of mitotic sister chromatid segregation (IDA)
Cellular Component
- Manually Curated
- located in astral microtubule (IMP)
- located in kinetochore (IDA)
- located in kinetochore microtubule (IDA)
- located in mitochondrion (HDA)
- located in spindle microtubule (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- CIN8/YEL061C is a non-essential gene; null mutant displays phenotypes associated with chromosome instability, such as abnormal chromosome segregation and cell cycle progression, and colony sectoring in a chromosome transmission fidelity (CTF) assay; null mutant cells are abnormally large, grow poorly, have increased mitophagy, and are sensitive to heat and various chemicals; heterozygous null mutant is haploinsufficient
Classical Genetics
- null
- cell cycle progression in anaphase: delayed
- cell cycle progression in metaphase: delayed
- chromosome segregation: abnormal
- chromosome/plasmid maintenance: abnormal
- colony sectoring: increased
- heat sensitivity: increased
- nuclear morphology: abnormal
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- resistance to chemicals: absent
- resistance to chemicals: decreased
- size of nucleolus: increased
- size of nucleus: increased
- spindle morphology: abnormal
- sporulation efficiency: decreased
- overexpression
- null
- cell size: increased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- competitive fitness: decreased
- desiccation resistance: decreased
- fermentative growth: decreased rate
- haploinsufficient
- heat sensitivity: decreased
- heat sensitivity: increased
- mitophagy: increased
- nuclear morphology: abnormal
- oxidative stress resistance: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- respiratory growth: increased rate
- size of nucleolus: increased
- stress resistance: decreased
- toxin resistance: decreased
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast CIN8 is homologous to human KIF11 and has been used to study pathogenesis of a subset of cancers, including colorectal, lung, prostate, breast, and uterine cancers
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Cin8p interacts physically with proteins involved in chromosome segregation; CIN8 interacts genetically with genes involved in mitotic cell cycle
719 total interactions for 345 unique genes
Physical Interactions
- Affinity Capture-MS: 38
- Affinity Capture-RNA: 7
- Affinity Capture-Western: 4
- Biochemical Activity: 4
- Co-localization: 1
- PCA: 2
- Proximity Label-MS: 1
- Reconstituted Complex: 5
- Two-hybrid: 13
Genetic Interactions
- Dosage Lethality: 2
- Dosage Rescue: 4
- Negative Genetic: 297
- Phenotypic Enhancement: 12
- Phenotypic Suppression: 17
- Positive Genetic: 14
- Synthetic Growth Defect: 73
- Synthetic Lethality: 211
- Synthetic Rescue: 14
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 6
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2025-02-03
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.