ARB1 / YER036C Overview
- Standard Name
- ARB1 1
- Systematic Name
- YER036C
- SGD ID
- SGD:S000000838
- Feature Type
- ORF , Verified
- Description
- ATPase of the ATP-binding cassette (ABC) family; involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; important for disaggregation of ordered and disordered aggregates; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p; human homolog ABCF2 can complement yeast ARB1 mutant 1 2 3
- Name Description
- ATP-binding cassette protein involved in Ribosome Biogenesis 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 610
- Mol. Weight (Da)
- 68370.8
- Isoelectric Point
- 6.47
- Median Abundance (molecules/cell)
- 32089 +/- 8268
- Half-life (hr)
- 9.5
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all ARB1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- ATPase involved in export of small ribosomal subunits from the nucleus and ribosome biogenesis; localizes in nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables ATP hydrolysis activity (IMP)
Biological Process
- Manually Curated
- acts upstream of or within protein unfolding (IMP)
- involved in ribosomal small subunit export from nucleus (IMP)
- involved in ribosome biogenesis (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Null mutants are inviable under standard aerobic conditions, viable but slow-growing under anaerobic conditions; reduced function mutants show decreased competitive fitness
Classical Genetics
- null
- reduction of function
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
165 total interactions for 140 unique genes
Physical Interactions
- Affinity Capture-MS: 131
- Affinity Capture-RNA: 10
- Affinity Capture-Western: 6
- Biochemical Activity: 5
- Co-localization: 1
- Co-purification: 1
- PCA: 1
- Proximity Label-MS: 2
- Reconstituted Complex: 1
- Two-hybrid: 1
Genetic Interactions
- Synthetic Growth Defect: 1
- Synthetic Lethality: 3
- Synthetic Rescue: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- ARB1 encodes an ATPase from the ATP-binding cassette (ABC) superfamily that is involved in ribosome biogenesis. Arb1p appears to be required for several steps in the biogenesis of both 40S and 60S ribosomal subunits, including the processing of 18S and 25S rRNA precursors in the nucleus, and the nuclear export of pre-40S and pre-60S particles. Consistent with its role in ribosome maturation, Arb1p shuttles between nucleus and cytoplasm. Also consistently, ARB1 is among the targets of Sfp1p transcription factor that regulates expression of ribosomal protein and biogenesis genes. It is proposed that the ATP-hydrolyzing activity of Arb1p provides a mechanochemical force required at multiple steps of ribosome biogenesis. Yet the exact mechanisms of Arb1p actions remain to be discovered, as do pathways that regulate its activity.
- Regulators
- 13
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.