VPH2 / YKL119C Overview
- Standard Name
- VPH2 1
- Systematic Name
- YKL119C
- SGD ID
- SGD:S000001602
- Aliases
- CLS10 6 , VMA12 6
- Feature Type
- ORF , Verified
- Description
- Integral membrane protein required for V-ATPase function; not an actual component of the vacuolar H+-ATPase (V-ATPase) complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER); involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner 1 2 3 4 5
- Name Description
- Vacuolar pH 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Vph2p is 215 amino acids long, short-lived, low in abundance
- Length (a.a.)
- 215
- Mol. Weight (Da)
- 25292.8
- Isoelectric Point
- 9.54
- Median Abundance (molecules/cell)
- 2843 +/- 1318
- Half-life (hr)
- 4.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all VPH2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Subunit of Vma12-Vma22 assembly complex, involved in vacuolar acidification and assembly of the V-type ATPase complex; localizes to ER membrane
View computational annotations
Molecular Function
- Manually Curated
- enables molecular function unknown (ND)
Biological Process
- Manually Curated
- involved in vacuolar acidification (IMP)
- involved in vacuolar proton-transporting V-type ATPase complex assembly (IMP)
Cellular Component
- Manually Curated
- located in endoplasmic reticulum (HDA)
- located in endoplasmic reticulum membrane (IDA)
- part of Vma12-Vma22 assembly complex (IPI)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- VPH2/YKL119C is a non-essential gene; null mutants are viable but exhibit a range of phenotypic changes including increased heat sensitivity, decreased metal and oxidative stress resistance, decreased acid pH resistance, auxotrophy, abnormal chromosome maintenance, decreased chronological lifespan, decreased competitive fitness, decreased desiccation resistance, decreased endocytosis, decreased freeze-thaw resistance, decreased hyperosmotic stress resistance, abnormal mitochondrial morphology, decreased resistance to enzymatic treatment, decreased respiratory growth rate, absent sporulation, decreased utilization rates of carbon and nitrogen sources, and decreased vegetative growth rate. Overexpression of VPH2 results in decreased acid pH resistance, decreased heat sensitivity, increased invasive growth, decreased vegetative growth rate, abnormal mitochondrial morphology, and increased replicative lifespan.
Classical Genetics
- null
- acid pH resistance: decreased
- auxotrophy
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chromosome/plasmid maintenance: abnormal
- chronological lifespan: decreased
- competitive fitness: decreased
- desiccation resistance: decreased
- endocytosis: decreased
- freeze-thaw resistance: decreased
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- metal resistance: decreased
- mitochondrial morphology: abnormal
- oxidative stress resistance: decreased
- protein/peptide modification: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- respiratory growth: absent
- respiratory growth: decreased rate
- respiratory growth: increased
- sporulation: absent
- utilization of carbon source: decreased
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- vegetative growth: decreased
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast VPH2 is homologous to human TMEM199, and has been used to study congenital disorder of glycosylation type IIp
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
311 total interactions for 238 unique genes
Physical Interactions
- Affinity Capture-MS: 7
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 3
- Co-purification: 13
- PCA: 2
- Two-hybrid: 1
Genetic Interactions
- Dosage Lethality: 1
- Negative Genetic: 199
- Phenotypic Enhancement: 2
- Phenotypic Suppression: 5
- Positive Genetic: 51
- Synthetic Growth Defect: 17
- Synthetic Lethality: 4
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 4
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.