CUP2 / YGL166W Overview
- Standard Name
- CUP2 1
- Systematic Name
- YGL166W
- SGD ID
- SGD:S000003134
- Aliases
- ACE1 2 6
- Feature Type
- ORF , Verified
- Description
- Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; required for regulation of copper genes in response to DNA-damaging reagents; CUP2 has a paralog, HAA1, that arose from the whole genome duplication 2 3 4 5
- Paralog
- HAA1 4
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- CUP2 has a paralog, HAA1, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 225
- Mol. Weight (Da)
- 24428.0
- Isoelectric Point
- 7.84
- Median Abundance (molecules/cell)
- 1453 +/- 641
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CUP2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Transcription factor that binds in a sequence-specific manner to DNA proximal to the RNA polymerase II core promoter; activates transcription of target genes in response to copper; localizes to the nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables copper ion binding (IDA)
- enables DNA-binding transcription factor activity, RNA polymerase II-specific (IMP, IDA)
- enables RNA polymerase II cis-regulatory region sequence-specific DNA binding (IDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in positive regulation of transcription by RNA polymerase II (IMP)
- involved in regulation of transcription by RNA polymerase II (IMP)
- involved in response to copper ion (IMP)
- involved in stress response to copper ion (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutation confers sensitivity to copper ions; in systematic studies, overexpression is lethal, whereas null mutation lowers competitive fitness
Classical Genetics
- reduction of function
- null
- unspecified
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The cup2 null mutant is viable; the null mutant of paralog haa1 is viable; the cup2 haa1 double mutant has not been annotated for phenotype.
96 total interactions for 91 unique genes
Physical Interactions
- Affinity Capture-MS: 2
- Affinity Capture-RNA: 8
- Affinity Capture-Western: 1
- FRET: 1
- Two-hybrid: 7
Genetic Interactions
- Dosage Lethality: 3
- Dosage Rescue: 1
- Negative Genetic: 58
- Phenotypic Enhancement: 1
- Phenotypic Suppression: 2
- Positive Genetic: 10
- Synthetic Rescue: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- CUP2 encodes a transcription factor that is a member of the copper fist family. In the presence of high copper levels, Cup2p activates transcription of genes involved in protection from copper toxicity, such as the metallothionein genes CUP1-1, CUP1-2, and CRS5, and the SOD1 gene encoding Cu,Zn superoxide dismutase. Cup2p is constitutively nuclear, and copper binding induces a conformational change allowing Cup2p to bind DNA. Once bound to the promoter, Cup2p recruits chromatin remodeling machinery and RNA polymerase II. CUP2 has a paralog, HAA1, which encodes a transcription factor involved in the response to weak acid stress.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.