RME1 / YGR044C Overview
- Standard Name
- RME1 1
- Systematic Name
- YGR044C
- SGD ID
- SGD:S000003276
- Aliases
- CSP1 5
- Feature Type
- ORF , Verified
- Description
- Zinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by a1-alpha2 regulator; mediates cell type control of sporulation; relocalizes from nucleus to cytoplasm upon DNA replication stress 2 3 4
- Name Description
- Regulator of MEiosis 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Relocalizes from nucleus to cytoplasm upon DNA replication stress
- Length (a.a.)
- 300
- Mol. Weight (Da)
- 33902.6
- Isoelectric Point
- 7.12
- Median Abundance (molecules/cell)
- 1441 +/- 441
- Half-life (min)
- 56.9
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all RME1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence-specific DNA-binding transcription factor; involved in negative regulation of transcription meiotic cell cycle genes; also involved in positive regulation of transcription of mitotic cell cycle genes during G1/S transition; localized to nucleus
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in G1/S transition of mitotic cell cycle (IDA)
- involved in meiotic cell cycle (IMP)
- involved in negative regulation of meiotic nuclear division (IMP)
- involved in negative regulation of transcription by RNA polymerase II (IDA, IMP)
- involved in positive regulation of invasive growth in response to glucose limitation (IDA)
- involved in positive regulation of transcription by RNA polymerase II (IMP, IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- overexpression
- null
- unspecified
- null
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
177 total interactions for 167 unique genes
Physical Interactions
- Affinity Capture-MS: 1
- Affinity Capture-RNA: 3
- Co-localization: 1
- Co-purification: 1
- PCA: 1
- Proximity Label-MS: 1
Genetic Interactions
- Dosage Lethality: 4
- Dosage Rescue: 7
- Negative Genetic: 135
- Phenotypic Suppression: 4
- Positive Genetic: 11
- Synthetic Growth Defect: 1
- Synthetic Haploinsufficiency: 1
- Synthetic Lethality: 3
- Synthetic Rescue: 3
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- RME1 encodes a transcription factor that is a member of the C2H2 zinc finger class. In haploid cells, Rme1p prevents the initiation of meiosis by negatively regulating IME1, encoding a master activator of meiosis. Rme1p also promotes mitosis, by positive regulation of the G1 cyclin gene CLN2. In addition, Rme1p has a major role in promoting filamentous growth via activation of transcription of FLO11, encoding a cell surface adhesin. Rme1p binds to Rme1 Response Elements, RREs, in the promoters of its target genes. Rme1p was thought to function as both a transcriptional activator, usually when bound close to its target promoter, and as a transcriptional repressor, when bound at a distance from its target gene. However, recent evidence suggests that Rme1p is always a transcriptional activator. Repression of IME1 transcription is accomplished by activation of transcription of a long noncoding RNA, IRT1, that overlaps the IME1 gene. Transcription of IRT1 recruits histone deacetylases to the IME1 gene and induces the formation of repressive chromatin across the region. By analogy, the repressive action of Rme1p at sites distant from the repressed target is now suggested to be caused by activation of noncoding transcription and subsequent chromatin repression. In diploids, transcription of RME1 is repressed by MATa1p-MATalpha2p repressor, causing the IRT1 ncRNA not to be transcribed and thereby allowing IME1 to be transcribed and meiosis to occur.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.