PUF3 / YLL013C Overview
- Standard Name
- PUF3 1
- Systematic Name
- YLL013C
- SGD ID
- SGD:S000003936
- Feature Type
- ORF , Verified
- Description
- Protein of the mitochondrial outer surface; links the Arp2/3 complex with the mitochondria during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins; translationally represses oxidative stress-linked genes; regulates CoQ biosynthesis 1 2 3 4 5
- Name Description
- PUmilio-homology domain Family 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- PUF3/YLL013C is located on the left arm of chromosome XII near the centromere; coding sequence is 2640 nucleotides long with a variable 5'-end and a variable repetitive region mid-gene
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Puf3p is 879 amino acids long, short-lived, low in abundance, with a variable N-terminus, and variable string of glutamines mid-protein; disordered regions in N-terminal half of protein; ubiquitinylated on K528, phosphorylated on 56 residues
- Length (a.a.)
- 879
- Mol. Weight (Da)
- 98064.9
- Isoelectric Point
- 7.22
- Median Abundance (molecules/cell)
- 4302 +/- 1035
- Half-life (hr)
- 5.0
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all PUF3 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- mRNA-binding protein involved in mRNA localization and deadenylation-dependent mRNA decay; also involved in mitochondrion organization and localization; localizes to the cytoplasm and the cytoplasmic side of the mitochondrial outer membrane
View computational annotations
Molecular Function
- Manually Curated
- enables mRNA binding (HDA, IDA)
Biological Process
- Manually Curated
- involved in aerobic respiration (IMP)
- involved in cellular response to glucose starvation (IMP)
- involved in intracellular mRNA localization (IMP)
- involved in mitochondrion localization (IMP)
- involved in mitochondrion organization (IMP)
- involved in nuclear-transcribed mRNA catabolic process (IDA)
- involved in nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay (IMP)
- involved in positive regulation of translation (IMP)
- involved in regulation of ubiquinone biosynthetic process (IMP)
Cellular Component
- Manually Curated
- located in cytoplasm (IDA)
- located in cytoplasmic side of mitochondrial outer membrane (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene in reference strain S288C; null mutant is viable and shows abnormal mitochondrial morphology and movement, increased innate thermotolerance, originless fragment maintenance (Ofm) phenotype, decreased hyperosmotic stress resistance, and sensitivity to various chemicals; both null mutation and overexpression confer respiratory growth defects
Classical Genetics
- null
- chromosome/plasmid maintenance: abnormal
- chronological lifespan: decreased
- chronological lifespan: increased
- competitive fitness: decreased
- competitive fitness: increased
- haploinsufficient
- hyperosmotic stress resistance: decreased
- innate thermotolerance: increased
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- starvation resistance: decreased
- stress resistance: decreased
- toxin resistance: decreased
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Puf3p interacts physically with proteins involved in transcription and rRNA processing; PUF3 interacts genetically with genes involved in mitotic cell cycle
3255 total interactions for 1986 unique genes
Physical Interactions
- Affinity Capture-MS: 59
- Affinity Capture-RNA: 3037
- Affinity Capture-Western: 11
- Co-crystal Structure: 1
- PCA: 2
- Protein-RNA: 6
- Reconstituted Complex: 1
- Two-hybrid: 2
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 1
- Negative Genetic: 104
- Phenotypic Enhancement: 2
- Phenotypic Suppression: 3
- Positive Genetic: 20
- Synthetic Growth Defect: 2
- Synthetic Lethality: 1
- Synthetic Rescue: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 9
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2025-11-13
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.