SIC1 / YLR079W Overview
- Standard Name
- SIC1 1
- Systematic Name
- YLR079W
- SGD ID
- SGD:S000004069
- Aliases
- SDB25 4 , BYC1 25
- Feature Type
- ORF , Verified
- Description
- Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylated by Clb5/6-Cdk1 and Cln1/2-Cdk1 kinase which regulate timing of Sic1p degradation; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 1 2 3 4 5 6 7 8 9 10
- Name Description
- Substrate/Subunit Inhibitor of Cyclin-dependent protein kinase 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 284
- Mol. Weight (Da)
- 32224.4
- Isoelectric Point
- 8.88
- Median Abundance (molecules/cell)
- 1499 +/- 1071
- Half-life (min)
- 36.5
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SIC1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Cyclin-dependent protein serine/threonine kinase inhibitor involved in G1/S transition of mitotic cell cycle and negative regulation of macroautophagy; localizes to both nucleus and cytoplasm
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in G1/S transition of mitotic cell cycle (IGI)
- involved in negative regulation of macroautophagy (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant displays a decreased growth rate, a reduced duration of the G1 phase of the cell cycle, is thermosensitive, and has impaired anaerobic growth during anoxia; null mutant has elevated levels of chromosome loss, and overall chromosomal instability; null mutant also displays a shorter chronological lifespan, accumulates superoxide, and is defective for entry into stationary phase; homozygous diploid null mutant has a sporulation defect, and reduced innate thermotolerance; overexpression enhances invasive growth
Classical Genetics
- null
- budding index: increased
- cell cycle progression through the G2/M phase transition: delayed
- chemical compound accumulation: increased
- chromosome/plasmid maintenance: abnormal
- chromosome/plasmid maintenance: decreased
- chronological lifespan: decreased
- colony sectoring: increased
- entry into G0 (stationary phase): decreased
- growth in exponential phase: decreased rate
- metal resistance: decreased
- mitotic cell cycle: abnormal
- protein/peptide distribution: abnormal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- telomere length: increased
- viable
- overexpression
- null
- anaerobic growth: decreased
- auxotrophy
- cell cycle progression in G1 phase: decreased duration
- chemical compound accumulation: abnormal
- chemical compound accumulation: increased
- chronological lifespan: decreased
- colony sectoring: increased
- competitive fitness: decreased
- endoplasmic reticulum morphology: abnormal
- haploproficient
- heat sensitivity: increased
- innate thermotolerance: decreased
- killer toxin resistance: decreased
- lipid particle morphology: abnormal
- mutation frequency: increased
- nuclear morphology: abnormal
- peroxisomal transport: decreased
- protein/peptide accumulation: increased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- respiratory growth: absent
- sporulation: decreased
- stress resistance: decreased
- transposable element transposition: increased
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- overexpression
- reduction of function
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
1180 total interactions for 717 unique genes
Physical Interactions
- Affinity Capture-MS: 33
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 15
- Biochemical Activity: 46
- Co-crystal Structure: 2
- Co-localization: 1
- FRET: 3
- PCA: 7
- Protein-peptide: 5
- Reconstituted Complex: 32
- Two-hybrid: 8
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 9
- Dosage Rescue: 29
- Negative Genetic: 667
- Phenotypic Enhancement: 24
- Phenotypic Suppression: 28
- Positive Genetic: 129
- Synthetic Growth Defect: 77
- Synthetic Lethality: 46
- Synthetic Rescue: 12
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 8
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2011-02-07
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.