GEP3 / YOR205C Overview
- Standard Name
- GEP3 1
- Systematic Name
- YOR205C
- SGD ID
- SGD:S000005731
- Aliases
- AIM40 2 , FMP38 , MTG3 3
- Feature Type
- ORF , Verified
- Description
- Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (Phb1p) 1 2 3
- Name Description
- GEnetic interactors of Prohibitins 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- GEP3/YOR205C is located on the right arm of chromosome XV between DED1 ATP-dependent DEAD-box RNA helicase and NOC2 ribosome biogenesis protein; coding sequence is 1671 nucleotides long with 16 SNPs, 9 of which cause amino acid polymorphisms
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Gep3p is 556 amino acids long, shorter-lived, extremely low in abundance; contains GTP-binding domain
- Length (a.a.)
- 556
- Mol. Weight (Da)
- 63878.3
- Isoelectric Point
- 9.71
- Median Abundance (molecules/cell)
- 985 +/- 524
- Half-life (hr)
- 6.8
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all GEP3 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Mitochondrial ribosome binding protein involved in SSU-rRNA maturation; localizes to mitochondrial inner membrane
View computational annotations
Molecular Function
- Manually Curated
- enables mitochondrial ribosome binding (IDA)
Biological Process
- Manually Curated
- involved in maturation of SSU-rRNA (IMP)
Cellular Component
- Manually Curated
- located in mitochondrial inner membrane (IDA)
- located in mitochondrion (HDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- GEP3/YOR205C is a non-essential gene in reference strain S288C; null mutants are viable, slow-growing, cannot respire or grow invasively, and have decreased chronological lifespan, decreased competitive fitness, decreased desiccation resistance, decreased filamentous growth, decreased heat resistance, abnormal mitochondrial and vacuoloar morphology, increased resistance to oxidative stress; overexpression slow vegetative growth, increases invasive growth
Classical Genetics
- null
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: decreased
- competitive fitness: decreased
- desiccation resistance: decreased
- filamentous growth: decreased
- haploinsufficient
- heat sensitivity: increased
- invasive growth: absent
- mitochondrial genome maintenance: absent
- mitochondrial morphology: abnormal
- oxidative stress resistance: increased
- protein/peptide accumulation: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: absent
- respiratory growth: decreased rate
- stress resistance: increased
- toxin resistance: increased
- utilization of carbon source: absent
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Gep3 interacts physically with proteins involved in mitochondrial translation; GEP3 interacts genetically with genes involved in transcription and lipid metabolism
534 total interactions for 458 unique genes
Physical Interactions
- Affinity Capture-MS: 12
- Affinity Capture-RNA: 6
- Co-fractionation: 1
- Co-localization: 1
- Proximity Label-MS: 3
Genetic Interactions
- Dosage Rescue: 1
- Negative Genetic: 362
- Positive Genetic: 135
- Synthetic Growth Defect: 10
- Synthetic Lethality: 2
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 3
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2025-11-11
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.