RPL36B / YPL249C-A Overview
- Standard Name
- RPL36B
- Systematic Name
- YPL249C-A
- SGD ID
- SGD:S000006438
- Aliases
- L36B 2 , L39 2 , YL39 2 , L36e 5 , eL36 17
- Feature Type
- ORF , Verified
- Description
- Ribosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication 1 2 3 4 5
- Name Description
- Ribosomal Protein of the Large subunit
- Paralog
- RPL36A 4
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- RPL36B is located on the left arm of chromosome XVI between autophagy gene ATG41 and GYP5 Rab GAP; dubious ORF YPL250W-A overlaps 3' end of RPL36B on the opposite strand; coding sequence is 541 nucleotides long with an intron at 17..254 and 6 SNPs, one of which causes a Thr/Ile polymorphism at residue 81; RPL36B has paralog RPL36A from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Rpl36bp is 100 amino acids long, of average half-life, high in abundance; acetylated on K86, phosphorylated on 5 residues, sumoylated on 4 lysines, ubiquitinylated on 2 lysines
- Length (a.a.)
- 100
- Mol. Weight (Da)
- 11155.6
- Isoelectric Point
- 12.14
- Median Abundance (molecules/cell)
- 36380 +/- 7430
- Half-life (hr)
- 9.7
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all RPL36B alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- RNA-binding subunit of the cytosolic large ribosomal subunit; involved in translation
View computational annotations
Molecular Function
- Manually Curated
- enables RNA binding (IDA)
- enables structural constituent of ribosome (IDA)
Biological Process
- Manually Curated
- involved in cytoplasmic translation (IDA)
Cellular Component
- Manually Curated
- part of cytosolic large ribosomal subunit (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null grows slowly, is sensitive to metals, has long G1, accumulates asparagine, lacks glutamate and proline; heterozygous null is haploinsufficient, shows chromosome instability; homozygous has small cell size, is sensitive to heat
Classical Genetics
- null
- acid pH resistance: decreased
- cell size: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chromosome/plasmid maintenance: decreased
- haploinsufficient
- heat sensitivity: increased
- metal resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- vegetative growth: decreased
- gain of function
- reduction of function
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Rpl36bp interacts physically with proteins involved in rRNA processing; RPL36B interacts genetically with genes involved in mitotic cell cycle; the rpl36b null mutant is viable, the null mutant of paralog rpl36a is viable, the rpl36b rpl36a double mutant is inviable.
184 total interactions for 161 unique genes
Physical Interactions
- Affinity Capture-MS: 156
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 1
- Co-purification: 2
- PCA: 1
- Proximity Label-MS: 1
- Two-hybrid: 5
Genetic Interactions
- Dosage Rescue: 4
- Negative Genetic: 1
- Phenotypic Enhancement: 1
- Phenotypic Suppression: 4
- Synthetic Growth Defect: 1
- Synthetic Lethality: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 15
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2007-02-14
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.