MBF1 / YOR298C-A Overview
- Standard Name
- MBF1 1
- Systematic Name
- YOR298C-A
- SGD ID
- SGD:S000007253
- Aliases
- SUF13
- Feature Type
- ORF , Verified
- Description
- Core integrated stress response (ISR) factor; optimizes ribosome collision-mediated Gcn2 activation and the downstream Gcn4-mediated transcriptional response; required for optimal stress-induced eIF2α (Sui2p) phosphorylation and downstream de-repression of GCN4 translation; Mbf1p is recruited to collided disomes and trisomes during the ISR; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress; homologous to human EDF1 2 3 4 5
- Name Description
- Multiprotein Bridging Factor 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Protein abundance increases in response to DNA replication stress
- Length (a.a.)
- 151
- Mol. Weight (Da)
- 16420.0
- Isoelectric Point
- 11.31
- Median Abundance (molecules/cell)
- 42645 +/- 26071
- Half-life (hr)
- 11.0
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all MBF1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein that interacts with colliding ribosome resulting in the activation of Gcn2p, leading to a robust integrated stress response; localizes to both the nucleus and cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- enables transcription coactivator activity (IDA)
Biological Process
- Manually Curated
- involved in positive regulation of transcription by RNA polymerase II (IDA)
Cellular Component
- Manually Curated
- located in mitochondrion (HDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- null
- overexpression
- reduction of function
- null
- acid pH resistance: decreased
- chemical compound accumulation: increased
- chromosome/plasmid maintenance: decreased
- competitive fitness: decreased
- heat sensitivity: decreased
- metal resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- starvation resistance: decreased
- stress resistance: decreased
- toxin resistance: decreased
- toxin resistance: increased
- viable
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
265 total interactions for 235 unique genes
Physical Interactions
- Affinity Capture-MS: 35
- Affinity Capture-RNA: 7
- Affinity Capture-Western: 2
- Biochemical Activity: 2
- Co-localization: 1
- Co-purification: 1
- Cross-Linking-MS (XL-MS): 2
- PCA: 2
- Protein-RNA: 1
- Proximity Label-MS: 6
- Reconstituted Complex: 3
- Two-hybrid: 1
Genetic Interactions
- Dosage Rescue: 1
- Negative Genetic: 158
- Phenotypic Enhancement: 2
- Phenotypic Suppression: 7
- Positive Genetic: 30
- Synthetic Growth Defect: 4
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- Mbf1p is a component of the integrated stress response that is recruited to colliding ribosomes. This ribosomal interaction results in the activation of Gcn2p, leading to eIF2α (Sui2p) phosphorylation and a downstream Gcn4p-mediated transcriptional response. In contrast to previous observations, MBF1 does not encode a transcriptional coactivator that mediates Gcn4p-dependent activation by bridging Gcn4p and Spt15p (TBP) and recruiting TBP to regulator bound promoters. The promoter of MBF1 contains a putative hypoxia response element (HRE).
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.