SAC1 / YKL212W Overview
- Standard Name
- SAC1 1
- Systematic Name
- YKL212W
- SGD ID
- SGD:S000001695
- Aliases
- RSD1 19
- Feature Type
- ORF , Verified
- Description
- Phosphatidylinositol phosphate phosphatase; role in early and medial Golgi PtdIns4P hydrolysis; regulated by Vps74p interaction; ER localized transmembrane protein which cycles through the Golgi; role in protein trafficking, processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis by modulating PtdIns(4)P metabolism; PtdIns4P PPase activity is crucial for autophagosome-vacuole fusion during autophagy; human homolog SACM1L complements autophagy defects of the null 2 3 4 5 7 8 9
- Name Description
- Suppressor of ACtin 6
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 623
- Mol. Weight (Da)
- 71125.2
- Isoelectric Point
- 7.8
- Median Abundance (molecules/cell)
- 10996 +/- 2810
- Half-life (hr)
- 11.2
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SAC1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Phosphatidylinositol phosphate phosphatase; involved in the hydrolysis of PI3P, PI4P, and PI(3,5)P2; localizes as an integral membrane protein to the endoplasmic reticulum, and Golgi apparatus
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- acts upstream of or within autophagosome-lysosome fusion (IMP)
- involved in phosphatidylinositol dephosphorylation (IMP, IGI, IDA)
Cellular Component
- Manually Curated
- located in cortical endoplasmic reticulum (IDA)
- located in endoplasmic reticulum (HDA)
- located in endoplasmic reticulum membrane (IDA, IMP)
- located in Golgi medial cisterna (IDA)
- located in Golgi membrane (IMP, IDA)
- located in host cell viral assembly compartment (IMP)
- located in mitochondrial outer membrane (HDA)
- located in mitochondrion (HDA)
- part of serine palmitoyltransferase complex (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Pathways
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutation affects phosphatidylinositol phosphate pools and leads to abnormal actin cytoskeleton, defects in protein secretion, endocytosis and vacuolar transport, increased sensitivity to low temperatures, cell wall-affecting drugs (caffeine, Calcofluor White) and multiple other chemicals; mutations cause inositol auxotrophy
Classical Genetics
- null
- acquired thermotolerance: normal
- actin cytoskeleton morphology: abnormal
- alkaline pH resistance: decreased
- autophagy: absent
- auxotrophy
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chitin deposition: abnormal
- cold sensitivity: increased
- critical cell size at START (G1 cell-size checkpoint): decreased
- endocytosis: delayed
- growth in exponential phase: decreased rate
- innate thermotolerance: decreased
- inviable
- ionic stress resistance: decreased
- killer toxin resistance: increased
- metal resistance: decreased
- protein secretion: decreased
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- septum formation: abnormal
- small molecule transport: decreased
- telomere length: decreased
- vacuolar morphology: abnormal
- vacuolar transport: delayed
- vegetative growth: decreased rate
- viable
- unspecified
- conditional
- actin cytoskeleton morphology: abnormal
- auxotrophy
- chemical compound accumulation: increased
- cold sensitivity: increased
- lipid particle morphology: abnormal
- mating efficiency: decreased
- protein secretion: abnormal
- resistance to chemicals: decreased
- sporulation: absent
- vacuolar morphology: abnormal
- vacuolar transport: normal
- null
- acid pH resistance: decreased
- cell size: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chemical compound excretion: increased
- chronological lifespan: decreased
- cold sensitivity: increased
- competitive fitness: decreased
- competitive fitness: increased
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- innate thermotolerance: decreased
- killer toxin resistance: decreased
- liquid culture appearance: abnormal
- metal resistance: decreased
- mitochondrial morphology: abnormal
- oxidative stress resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- respiratory growth: absent
- stress resistance: decreased
- toxin resistance: decreased
- utilization of carbon source: decreased
- utilization of iron source: decreased
- utilization of nitrogen source: decreased rate
- utilization of phosphorus source: decreased
- UV resistance: decreased
- vacuolar morphology: abnormal
- vegetative growth: decreased
- vegetative growth: decreased rate
- viable
- overexpression
- unspecified
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
1664 total interactions for 1096 unique genes
Physical Interactions
- Affinity Capture-MS: 77
- Affinity Capture-RNA: 3
- Affinity Capture-Western: 9
- Biochemical Activity: 2
- Co-crystal Structure: 1
- Co-localization: 1
- Co-purification: 6
- Far Western: 1
- PCA: 12
- Proximity Label-MS: 1
- Reconstituted Complex: 2
- Two-hybrid: 4
Genetic Interactions
- Dosage Lethality: 2
- Dosage Rescue: 15
- Negative Genetic: 1200
- Phenotypic Enhancement: 13
- Phenotypic Suppression: 10
- Positive Genetic: 213
- Synthetic Growth Defect: 40
- Synthetic Haploinsufficiency: 1
- Synthetic Lethality: 34
- Synthetic Rescue: 17
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 6
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2008-06-26
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.