AMN1 / YBR158W Overview
- Standard Name
- AMN1 1
- Systematic Name
- YBR158W
- SGD ID
- SGD:S000000362
- Aliases
- CST13 2 , ICS4 4
- Feature Type
- ORF , Verified
- Description
- Modulator of cell separation and mitotic exit; inhibits separation through Ub-dependent Ace2p proteolysis; part of a daughter-specific switch induced by the mitotic exit network that inhibits exit and resets the cell cycle after the execution of MEN function, blocking Tem1p and Cdc15 association; required for chromosome stability and multiple mitotic checkpoints; regulated by SCF; haploid transcription regulated by Ste12p; contains 12 degenerate leucine-rich repeat motifs and an atypical F-box 1 2 3
- Name Description
- Antagonist of Mitotic exit Network 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 549
- Mol. Weight (Da)
- 62703.2
- Isoelectric Point
- 9.53
- Median Abundance (molecules/cell)
- 1682 +/- 848
- Half-life (min)
- 23.1
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all AMN1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Negatively regulates mitotic exit by binding to the Tem1p GTPase and diassembling the Tem1p-Cdc15p complex; inhibits cell separation through SCF, ubiquitin-dependent proteolysis of Ace2p; involved in the mitotic cell cycle checkpoint; localizes to the nucleus, cytoplasm, and cellular bud
View computational annotations
Molecular Function
- Manually Curated
- enables small GTPase binding (IPI)
Biological Process
- Manually Curated
- involved in mitotic spindle assembly checkpoint signaling (IMP)
- involved in mitotic spindle orientation checkpoint signaling (IMP)
- involved in negative regulation of exit from mitosis (IGI, IMP)
- involved in negative regulation of septum digestion after cytokinesis (IGI, IMP)
- involved in protein-containing complex disassembly (IMP)
- involved in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant has slow growth in rich media, decreased competitive fitness in minimal medium and a reduced ability to utilize urea as nitrogen source; null mutant displays greatly decreased filamentous growth and is unable to undergo invasive growth; null mutant displays reduced flocculation; overexpression results in a decreased growth rate, misshapen, elongated cells with multiple nuclei and a shift towards 2C DNA content with eventual inviability; overexpression results in an elevated frequency of mitotic recombination, and an increased frequency of chromosome loss and non-disjunction, indicative of chromosomal instability
Classical Genetics
- null
- flocculation: decreased
- growth in exponential phase: increased rate
- liquid culture appearance: abnormal
- liquid culture appearance: normal
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- protein/peptide modification: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- vegetative growth: decreased
- viable
- overexpression
- reduction of function
- null
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
101 total interactions for 74 unique genes
Physical Interactions
- Affinity Capture-MS: 6
- Affinity Capture-RNA: 10
- Affinity Capture-Western: 10
- Co-purification: 1
- Reconstituted Complex: 1
- Two-hybrid: 1
Genetic Interactions
- Dosage Lethality: 18
- Dosage Rescue: 1
- Negative Genetic: 39
- Phenotypic Suppression: 3
- Positive Genetic: 4
- Synthetic Lethality: 1
- Synthetic Rescue: 6
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.