FZO1 / YBR179C Overview
- Standard Name
- FZO1 1
- Systematic Name
- YBR179C
- SGD ID
- SGD:S000000383
- Feature Type
- ORF , Verified
- Description
- Mitofusin, involved in mitochondrial outer membrane fusion; mediates contacts between mitochondria and peroxisomes that regulate mitochondrial fusion; GTPase domain is required for auto-oligomerization and for efficient tethering, homotypic fusion and degradation of Fzo1p; role in mitochondrial genome maintenance; targeted for destruction by SCF-Mdm30p and the cytosolic ubiquitin-proteasome system; activity regulated by ubiquitination at conserved lysines and by deubiquitinases Ubp2p and Ubp12p 1 2 3 5 6 7 8 9 10 11
- Name Description
- FuZzy Onions homolog 4
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Mitofusin Fzo1p is 855 amino acids long, short-lived, and low in abundance; phosphorylated on 3 serines, ubiquitinylated on 5 lysines; shares domains with other proteins involved in organelle fission and mitochondrion organization
- Length (a.a.)
- 855
- Mol. Weight (Da)
- 97804.9
- Isoelectric Point
- 7.0
- Median Abundance (molecules/cell)
- 1772 +/- 495
- Half-life (hr)
- 3.0
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all FZO1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- GTPase involved in mitochondrial fusion as a homodimer; localizes to both mitochondrial inner and outer membranes
View computational annotations
Molecular Function
- Manually Curated
- enables GTPase activity (IDA)
- enables peroxisome-mitochondrion membrane tether activity (IDA, IMP)
Biological Process
- Manually Curated
- acts upstream of or within intracellular distribution of mitochondria (IGI)
- involved in mitochondrial fusion (IMP, IPI)
Cellular Component
- Manually Curated
- located in mitochondrial outer membrane (IDA, HDA)
- located in mitochondrion (HDA)
- is active in peroxisomal-mitochondrial contact site (IDA)
- is active in peroxisome (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant has fragmented mitochondria, loses the mitochondrial genome, and cannot grow on nonfermentable carbon sources; overexpression also causes mitochondrial fragmentation; null mutant is sensitive to hyperosmotic stress and has lengthened replicative lifespan but shortened chronological lifespan; in large-scale studies, the null mutant has reduced competitive fitness, poor utilization of several nitrogen sources, and altered resistance to various chemicals
Classical Genetics
- overexpression
- null
- chemical compound accumulation: increased
- desiccation resistance: decreased
- hyperosmotic stress resistance: decreased
- mitochondrial distribution: abnormal
- mitochondrial genome maintenance: absent
- mitochondrial genome maintenance: decreased
- mitochondrial morphology: abnormal
- mitophagy: decreased
- petite
- replicative lifespan: increased
- respiratory growth: absent
- respiratory metabolism: decreased
- vegetative growth: decreased
- repressible
- conditional
- reduction of function
- unspecified
- null
- biofilm formation: decreased
- biofilm formation: increased
- cell cycle progression in G1 phase: increased duration
- cell size: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chronological lifespan: decreased
- chronological lifespan: increased
- competitive fitness: decreased
- desiccation resistance: decreased
- haploinsufficient
- heat sensitivity: increased
- innate thermotolerance: decreased
- mitochondrial genome maintenance: absent
- mitochondrial genome maintenance: decreased
- mitochondrial morphology: abnormal
- mutation frequency: increased
- oxidative stress resistance: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: absent
- respiratory growth: decreased rate
- toxin resistance: decreased
- utilization of carbon source: decreased
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- vegetative growth: absent
- vegetative growth: decreased
- vegetative growth: decreased rate
- viable
- overexpression
- unspecified
- repressible
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- FZO1 is homologous to human MFN2, and has been used to study Charcot-Marie-Tooth disease type 2A (CMT2A)
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Fzo1p interacts physically with proteins involved in mitochondrion organization; FZO1 interacts genetically with genes involved in mitochondrion organization
229 total interactions for 159 unique genes
Physical Interactions
- Affinity Capture-MS: 11
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 30
- Biochemical Activity: 1
- Co-fractionation: 3
- Reconstituted Complex: 1
- Two-hybrid: 1
Genetic Interactions
- Dosage Lethality: 1
- Dosage Rescue: 10
- Negative Genetic: 75
- Phenotypic Enhancement: 8
- Phenotypic Suppression: 18
- Positive Genetic: 26
- Synthetic Growth Defect: 10
- Synthetic Haploinsufficiency: 1
- Synthetic Lethality: 8
- Synthetic Rescue: 19
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- FZO1 promoter is bound by Gcn5p, Med6p, Rgr1p, Uga3p, Xbp1p, and Yap5p in response to heat; Fzo1p protein stability is downregulated by Mdm30p, Rsp5p, and Ubp12p; Fzo1p protein stability is upregulated by Ubp2p; Fzo1p protein stability is down regulated by Doa1p during replicative senescence
- Regulators
- 15
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-06-14
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.