RSP5 / YER125W Overview
- Standard Name
- RSP5 1
- Systematic Name
- YER125W
- SGD ID
- SGD:S000000927
- Aliases
- UBY1 , MDP1 28 , MUT2 29 , NPI1 30 , SMM1 31 , HPG1 32
- Feature Type
- ORF , Verified
- Description
- NEDD4 family E3 ubiquitin ligase; regulates processes including: MVB sorting, the heat shock response, transcription, endocytosis and ribosome stability; ubiquitinates Sec23p, Sna3p, Ste4p, Nfi1p, Rpo21p, Sem1p, Dip5p and Put4p; autoubiquitinates; deubiquitinated by Ubp2p; regulated by SUMO ligase Siz1p, in turn regulates Siz1p SUMO ligase activity; required for efficient Golgi-to-ER trafficking in COPI mutants; mutant tolerates aneuploidy; human homolog implicated in Liddle syndrome 2 3 4 5 6 7 9 10 11 12 13 14 15 16 17 18 19 20
- Name Description
- Reverses Spt- Phenotype 8
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 809
- Mol. Weight (Da)
- 91810.2
- Isoelectric Point
- 6.67
- Median Abundance (molecules/cell)
- 6335 +/- 1139
- Half-life (hr)
- 7.8
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all RSP5 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- E3 ubiquitin ligase that conjugates ubiquitin to proteins to target them for degradation; known to ubiquitinate Ste4p; involved in regulating a variety of processes via ubiquitin-dependent catabolism including organization of the mitochondrion, multivesilular body size, initiation of the mating tip projection and actin cytoskeletal organization; positively regulates dolichol and fatty acid biosynthesis; localized throughout the cell
View computational annotations
Molecular Function
- Manually Curated
- enables phosphatidylinositol binding (IDA)
- enables ubiquitin binding (IDA)
- enables ubiquitin protein ligase activity (IDA)
- enables ubiquitin-protein transferase activity (IDA, IMP)
- enables ubiquitin-ubiquitin ligase activity (IDA)
Biological Process
- Manually Curated
- involved in cellular response to amino acid stimulus (IMP)
- involved in cellular response to heat (IMP)
- involved in cellular response to L-arginine (IMP)
- involved in cellular response to stress (IMP)
- involved in chromatin organization (IMP)
- involved in free ubiquitin chain polymerization (IDA)
- involved in late endosome to vacuole transport via multivesicular body sorting pathway (IPI, IMP)
- involved in mitochondria-associated ubiquitin-dependent protein catabolic process (IMP)
- involved in mitochondrion organization (IGI, IMP)
- involved in nonfunctional rRNA decay (IDA)
- involved in poly(A)+ mRNA export from nucleus (IMP)
- involved in positive regulation of endocytosis (IMP)
- involved in positive regulation of fatty acid biosynthetic process (IMP)
- involved in positive regulation of proteasomal ubiquitin-dependent protein catabolic process (IMP)
- involved in positive regulation of receptor-mediated endocytosis (IMP)
- involved in positive regulation of transcription by RNA polymerase II (IMP)
- involved in proteasome-mediated ubiquitin-dependent protein catabolic process (IPI)
- involved in protein K63-linked ubiquitination (IDA)
- involved in protein quality control for misfolded or incompletely synthesized proteins (IDA)
- involved in protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway (IMP)
- involved in protein ubiquitination (IDA, IMP)
- involved in regulation of actin cytoskeleton organization (IGI)
- involved in regulation of dolichol biosynthetic process (IMP, IGI)
- involved in regulation of ergosterol biosynthetic process (IMP, IGI)
- involved in regulation of mRNA export from nucleus (IPI, IMP)
- involved in regulation of nitrogen utilization (IGI)
- involved in regulation of phosphate metabolic process (IGI)
- involved in regulation of protein localization (IMP, IPI)
- involved in regulation of ribosomal large subunit export from nucleus (IMP)
- involved in regulation of rRNA processing (IMP)
- involved in regulation of tRNA export from nucleus (IMP)
- involved in regulation of tRNA processing (IMP)
- involved in regulation of ubiquinone biosynthetic process (IGI, IMP)
- involved in ribophagy (IGI)
- involved in ubiquitin-dependent endocytosis (IMP)
- involved in ubiquitin-dependent protein catabolic process (IMP)
- involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway (IMP)
Cellular Component
- Manually Curated
- located in cellular bud tip (IDA)
- located in cytoplasm (IDA)
- is active in cytosol (IMP)
- is active in cytosolic ribosome (IDA)
- located in endosome membrane (IDA)
- located in Golgi apparatus (IDA)
- located in mitochondrion (HDA)
- located in nucleus (IDA)
- located in plasma membrane (HDA)
- part of ubiquitin ligase complex (IPI)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; conditional (ts) mutations lead to buildup of unprocessed reporter proteins normally targeted for degradation and to defects in processing of mRNA, rRNA and tRNA precursors; reduction of function causes higher sensitivity to cell wall-affecting chemicals (caffeine, Calcofluor White); repression results in opi- phenotype (overproduction and excretion of inositol in the absence of inositol and choline); overexpression causes slow growth
Classical Genetics
- overexpression
- repressible
- reduction of function
- conditional
- chemical compound accumulation: increased
- endocytosis: absent
- heat sensitivity: increased
- innate thermotolerance: decreased
- nuclear export: decreased
- oxidative stress resistance: decreased
- protein/peptide accumulation: abnormal
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- protein/peptide modification: absent
- protein/peptide modification: decreased
- resistance to chemicals: decreased
- RNA accumulation: abnormal
- RNA accumulation: decreased
- RNA accumulation: increased
- toxin resistance: decreased
- vegetative growth: decreased
- unspecified
- null
- overexpression
- reduction of function
- repressible
- conditional
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast RSP5 is orthologous to human NEDD4 and has been used to study Liddle syndrome
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
1984 total interactions for 1260 unique genes
Physical Interactions
- Affinity Capture-MS: 176
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 86
- Biochemical Activity: 160
- Co-crystal Structure: 2
- Co-fractionation: 2
- Co-localization: 2
- Co-purification: 1
- Far Western: 2
- PCA: 2
- Protein-peptide: 125
- Proximity Label-MS: 2
- Reconstituted Complex: 132
- Two-hybrid: 35
Genetic Interactions
- Dosage Growth Defect: 6
- Dosage Lethality: 17
- Dosage Rescue: 29
- Negative Genetic: 978
- Phenotypic Enhancement: 29
- Phenotypic Suppression: 21
- Positive Genetic: 129
- Synthetic Growth Defect: 22
- Synthetic Lethality: 6
- Synthetic Rescue: 16
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2010-02-01
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.