UPC2 / YDR213W Overview
- Standard Name
- UPC2 1
- Systematic Name
- YDR213W
- SGD ID
- SGD:S000002621
- Aliases
- MOX4 6
- Feature Type
- ORF , Verified
- Description
- Sterol regulatory element binding protein; induces sterol biosynthetic genes, upon sterol depletion; acts as a sterol sensor, binding ergosterol in sterol rich conditions; relocates from intracellular membranes to perinuclear foci upon sterol depletion; redundant activator of filamentation with ECM22, up-regulating the expression of filamentous growth genes; contains a Zn[2]-Cys[6] binuclear cluster; UPC2 has a paralog, ECM22, that arose from the whole genome duplication 2 3 4 5 6 7 8 9 10 11 12 13
- Name Description
- UPtake Control 1 3
- Paralog
- ECM22 10
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- UPC2 has a paralog, ECM22, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 913
- Mol. Weight (Da)
- 100324.8
- Isoelectric Point
- 5.86
- Median Abundance (molecules/cell)
- 746 +/- 427
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all UPC2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence-specific DNA-binding RNA polymerase II transcription activator involved in positive regulation of ergosterol biosynthesis, sterol import, and filamentous growth in response to starvation; localizes to membranes, the perinuclear region of the cytoplasm, and the nucleus
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in cellular response to hypoxia (IMP)
- involved in positive regulation of ergosterol biosynthetic process (IMP)
- involved in positive regulation of filamentous growth of a population of unicellular organisms in response to starvation (IGI, IMP)
- involved in positive regulation of sterol import (IMP)
- involved in positive regulation of transcription by RNA polymerase II (IMP, IGI)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- null
- overexpression
- unspecified
- gain of function
- null
- overexpression
- reduction of function
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The upc2 null mutant is viable; the null mutant of paralog ecm22 is viable; the upc2 ecm22 double mutant displays a synthetic growth defect.
174 total interactions for 135 unique genes
Physical Interactions
- Affinity Capture-MS: 12
- Affinity Capture-RNA: 5
- Co-localization: 1
- Reconstituted Complex: 1
- Two-hybrid: 2
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 2
- Negative Genetic: 88
- Phenotypic Enhancement: 10
- Phenotypic Suppression: 22
- Positive Genetic: 15
- Synthetic Growth Defect: 3
- Synthetic Lethality: 10
- Synthetic Rescue: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- UPC2 encodes a transcription factor that is a member of the C6 zinc finger class, containing a DNA binding domain also known as the Zn2Cys6 binuclear zinc cluster or zinc knuckle. Upc2p and Ecm22p, the products of paralogous genes, have similar functions. They recognize and bind to sterol regulatory elements (SRE) in the promoters of genes involved in ergosterol biosynthesis, such as ERG1, ERG2, ERG3, ERG7, ERG25, ERG26, and ERG27, upregulating their transcription at low sterol levels. Under hypoxic conditions, both Upc2p and Ecm22p also activate transcription of the DAN/TIR genes encoding cell wall mannoproteins. Upc2p also activates transcription of the AUS1 and PDR11 genes encoding transporters that mediate sterol uptake. Under sterol-replete conditions, Upc2p and Ecm22p localize to intracellular membranes. When sterol concentrations are low, both transcription factors relocate to the nucleus to activate ergosterol biosynthetic gene transcription. By analogy to sterol biosynthesis regulators in other organisms, it is likely that Upc2p and Ecm22p are processed during this relocation, such that the C-terminal transmembrane domain remains in the membrane while the N-terminal DNA-binding domain enters the nucleus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.