REA1 / YLR106C Overview
- Standard Name
- REA1 6
- Systematic Name
- YLR106C
- SGD ID
- SGD:S000004096
- Aliases
- MDN1 2
- Feature Type
- ORF , Verified
- Description
- Huge dynein-related AAA-type ATPase (midasin); forms extended pre-60S particle with the Rix1 complex; involved with interaction partners Rsa4p and Ytm1p, in the ATP-dependent remodeling of the pre-60S particle at successive maturation steps during ribosomal biogenesis; involved in the removal of biogenesis factors including GTPase Nog2p prior to nuclear export; contains a hexameric AAA-motor head domain and a long flexible tail with a MIDAS (metal ion-dependent adhesion site) domain 1 2 3 4 5
- Name Description
- Ribosome Export/Assembly 6
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- REA1 is located on the right arm of chromosome XII between SNR79 C/D box snoRNA and REX3 RNA exonuclease; at 14733 nucleotides, REA1 has the longest coding sequence in the genome, with 62 synonymous SNPs, 38 amino acid polymorphisms, and a 21-nucleotide in-frame deletion in Σ1278b near the C-terminal end at nucleotide 12906
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Midasin Rea1p is 4910 amino acids long, somewhat shorter-lived, low in abundance; abundance increases when treated with methyl methanesulfonate; contains 8 AAA ATPase domains, 4 AAA lid domains; phosphorylated on 32 residues, ubiquitinylated on 3 lysines; protein sequence is 4903 amino acids in Σ1278b due to 7-aa in-frame deletion near C-terminus
- Length (a.a.)
- 4910
- Mol. Weight (Da)
- 559077.9
- Isoelectric Point
- 4.63
- Median Abundance (molecules/cell)
- 3876 +/- 2053
- Half-life (hr)
- 8.7
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all REA1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- An ATPase involved in ATP-dependent remodeling of the pre-60S particle, regulating export from the nucleus; localizes to nucleoplasm
View computational annotations
Molecular Function
- Manually Curated
- enables ATP hydrolysis activity (IDA, IMP)
Biological Process
- Manually Curated
- involved in protein-RNA complex remodeling (IMP, IDA)
- involved in regulation of ribosomal subunit export from nucleus (IMP)
- involved in ribosomal large subunit assembly (IMP)
- involved in rRNA processing (IMP)
Cellular Component
- Manually Curated
- located in mitochondrion (HDA)
- located in nucleoplasm (IDA)
- located in nucleus (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; repressible alleles reveal defects in rRNA processing and ribosome biogenesis; reduction of function alleles cause slow growth, long telomeres, decreased competitive fitness; heterozygous null mutants show elevated global mutation frequency and genetic instability; conditional alleles increase heat sensitivity, cause defects in rRNA processing and ribosome biogenesis
Classical Genetics
- repressible
- null
- reduction of function
- conditional
- null
- reduction of function
- repressible
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Rea1p interacts physically with proteins involved in rRNA processing; REA1 interacts genetically with genes involved in mRNA processing
266 total interactions for 184 unique genes
Physical Interactions
- Affinity Capture-MS: 155
- Affinity Capture-RNA: 11
- Affinity Capture-Western: 11
- Co-crystal Structure: 1
- Co-localization: 1
- Co-purification: 3
- Proximity Label-MS: 1
- Reconstituted Complex: 6
- Two-hybrid: 12
Genetic Interactions
- Negative Genetic: 61
- Positive Genetic: 3
- Synthetic Growth Defect: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 5
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.