ADI1 / YMR009W Overview
- Standard Name
- ADI1 1
- Systematic Name
- YMR009W
- SGD ID
- SGD:S000004611
- Feature Type
- ORF , Verified
- Description
- Acireductone dioxygenease involved in methionine salvage pathway; transcribed as polycistronic mRNA with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions; human ortholog ADI1 can complement yeast adi1 mutant 1 2
- Name Description
- Acireductone DIoxygenase 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 179
- Mol. Weight (Da)
- 20870.5
- Isoelectric Point
- 4.98
- Median Abundance (molecules/cell)
- 2967 +/- 1243
- Half-life (hr)
- 4.8
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all ADI1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Nickel-requiring acireductone dioxygenase involved in the regeneration of methionine from methylthioadenosine; localizes to both the nucleus and cytoplasm
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in L-methionine salvage from methylthioadenosine (IMP, ISS, IGI)
Pathways
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutants show increased competitive fitness, have abnormal vacuolar morphology, are sensitive to UV and to desiccation; heterozygous diploid nulls are haploinsufficient
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
124 total interactions for 100 unique genes
Physical Interactions
- Affinity Capture-MS: 3
- Affinity Capture-RNA: 6
- Proximity Label-MS: 1
- Reconstituted Complex: 1
- Two-hybrid: 1
Genetic Interactions
- Negative Genetic: 82
- Positive Genetic: 28
- Synthetic Growth Defect: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- ADI1 encodes acireductone dioxygenease, a key enzyme of the methionine salvage pathway, which regenerates methionine from 5'-methylthioadenosine, a byproduct of metabolic reactions involving S-adenosylmethionine. The ADI1 gene is transcribed into at least five mRNA species, including a dicistronic transcript that also covers the downstream gene YMR10W. The transcription is generally induced under heat shock conditions, but one of the transcripts with a shorter 5' end is not, suggesting a presence of an alternative promoter that is not heat-responsive. The steady-state level of ADI1 mRNA is controlled post-transcriptionally by RNase III encoded by RNT1. Rnt1p recognizes and cleaves at a stem loop structure formed between nucleotides 324 and 394 of the ADI1 ORF and the cleavage products become subject to degradation by nuclear exonucleases Xrn1p, Rat1p and Rrp6p. The role of Rnt1p in the ADI1 regulation appears to be in preventing the accumulation of ADI1 mRNA and in the removal of aberrant, 3'-extended mRNAs that are caused by inefficient 3'-end processing signal of the ADI1 gene. Human ortholog ADI1/MTCBP-1/ARD, whose overexpression inhibits proliferation of prostate cancer cell, can complement yeast adi1 mutant.
- Regulators
- 15
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.