MCM1 / YMR043W Overview
- Standard Name
- MCM1 1 2 3
- Systematic Name
- YMR043W
- SGD ID
- SGD:S000004646
- Aliases
- FUN80 9
- Feature Type
- ORF , Verified
- Description
- Transcription factor; involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes; relocalizes to the cytosol in response to hypoxia 4 5 6 8
- Name Description
- MiniChromosome Maintenance 7
- Paralog
- ARG80
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Relocalizes to the cytosol in response to hypoxia
- Length (a.a.)
- 286
- Mol. Weight (Da)
- 32787.0
- Isoelectric Point
- 4.77
- Median Abundance (molecules/cell)
- 3789 +/- 1265
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all MCM1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence-specific DNA-binding RNA polymerase II transcription activator and repressor; involved in the regulation of mating type switching, mating-type specific transcription, arginine metabolism, and G2/M transition of mitotic cell cycle; binds DNA replication origins and is involved in DNA replication initiation; localizes to the cytosol, nucleus, and nuclear chromatin
View computational annotations
Molecular Function
- Manually Curated
- enables DNA replication origin binding (IDA)
- enables DNA-binding transcription activator activity, RNA polymerase II-specific (IDA, IMP)
- enables DNA-binding transcription factor activity, RNA polymerase II-specific (IDA, IPI)
- enables DNA-binding transcription repressor activity, RNA polymerase II-specific (IDA)
- enables RNA polymerase II cis-regulatory region sequence-specific DNA binding (IDA)
- enables RNA polymerase II cis-regulatory region sequence-specific DNA binding, bending (IDA, IMP)
- enables RNA polymerase II-specific DNA-binding transcription factor binding (IMP, IPI, IDA)
- enables sequence-specific DNA binding (HDA)
Biological Process
- Manually Curated
- involved in G2/M transition of mitotic cell cycle (IMP)
- involved in negative regulation of arginine catabolic process (IMP)
- involved in negative regulation of transcription by RNA polymerase II (IMP, IDA)
- involved in positive regulation of arginine biosynthetic process (IMP, IDA)
- involved in positive regulation of transcription by RNA polymerase II (IMP, IDA)
- involved in regulation of mating type switching (IMP)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; identified as an ARS-specific minichromosome maintenance mutant; reduction of function mutant is an alpha-specific sterile with decreased pheromone-induced cell cycle arrest, defective alpha factor production, decreased growth rate, reduced viability, elongated and misshapen buds, and abnormal spindle morphology; temperature sensitive mutant arrests in mitosis with large, elongated buds, a single, undivided nucleus in or near the neck, and has abnormal nuclear morphology and chromosomal instability; overexpression results in G1 arrest with some elongated and pear-shaped cells; heterozygous null is haploinsufficient
Classical Genetics
- overexpression
- repressible
- null
- reduction of function
- alkaline pH resistance: decreased
- bud morphology: abnormal
- chromosome/plasmid maintenance: decreased
- growth in exponential phase: decreased rate
- growth in exponential phase: normal rate
- mating efficiency: decreased
- pheromone production: decreased
- pheromone-induced cell cycle arrest: decreased
- resistance to chemicals: decreased
- spindle morphology: abnormal
- sterile
- viability: decreased
- viability: normal
- conditional
- bud morphology: abnormal
- cell cycle progression in M phase: arrested
- chromosome/plasmid maintenance: abnormal
- chromosome/plasmid maintenance: decreased
- colony sectoring: increased
- heat sensitivity: increased
- mating response: abnormal
- mitotic recombination: increased
- nuclear morphology: abnormal
- vegetative growth: decreased rate
- null
- reduction of function
- overexpression
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
444 total interactions for 412 unique genes
Physical Interactions
- Affinity Capture-MS: 14
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 9
- Co-crystal Structure: 2
- Far Western: 1
- PCA: 1
- Reconstituted Complex: 15
- Two-hybrid: 13
Genetic Interactions
- Dosage Growth Defect: 2
- Dosage Rescue: 8
- Negative Genetic: 255
- Phenotypic Enhancement: 2
- Phenotypic Suppression: 6
- Positive Genetic: 106
- Synthetic Lethality: 3
- Synthetic Rescue: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- MCM1 encodes an alpha helix transcription factor of the MADS box family. Mcm1p binds 5'-CCNNWTYRGGAA-3' motifs, and is involved in the control of various cellular processes such as argnine metabolism, M/G1 and G2/M cell-cycle-dependent transcription, recombination, TY transcription and osmotolerance. Mcm1p regulates cell-type specification by binding target sites upstream of the promoters of nine a-specific genes (MFA1, MFA2, STE2, STE6, BAR1, AGA2, DPS1, ASG7, DPS2) to activate their transcription. In alpha cells, Mcm1p combines with MATalpha1p to activate transcription of at least four alpha-specific genes (MFalpha1, MFalpha2, STE3, SAG1). Repression of a-specific genes in alpha haploid or alpha/a diploid cells involves cooperative binding of MATalpha2p and Mcm1p. In cell-cycle control, Mcm1p regulates two consecutive waves of transcription controlling G1/S and G2/M cell-cycle transitions. Transcription of four genes (CLN3, SWI4, CDC6, CDC47) peaks in late M-early G1 phase of the cell cycle via an early cell-cycle box (ECB) bound constitutively by Mcm1p. Mcm1p also controls expression of genes involved in pre-replication complex (PRC) formation, and binds cooperatively to multiple sites at autonomously replicating sequences. Transcription of 26 genes, called the CLB2 cluster, peaks in late G2/early M phase of the cell cycle and requires Mcm1p. Mcm1p is also required for the repression of arginine biosynthetic genes (ARG1, ARG3, ARG5,6 and ARG8) and induction of arginine catabolic genes (CAR1 and CAR2) in response to arginine. Regulation of arginine-dependent gene expression is mediated by the multimeric ArgR/Mcm1 complex that binds upstream regulatory motifs in the promoters of arginine-sensitive promoters. The ArgR/Mcm1 complex consists of Arg80p, Arg81p, Arg82p, and Mcm1p. Arg80p and Mcm1p form heterodimers and coregulate gene expression by facilitating the cooperative binding of diverse sequence-specific factors to promoters. During arginine starvation, Mcm1p positively regulates ARG1 transcription by enhancing binding of Gcn4p to the ARG1 promoter and recruiment of the SWI/SNF chromatin-remodeling complex. Mcm1p is phosphorylated on two major phosphorylation sites in its N-terminus. Mcm1p activity may be regulated by the SLN1 signaling pathway.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-11-13
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.