FAP1 / YNL023C Overview
- Standard Name
- FAP1 1
- Systematic Name
- YNL023C
- SGD ID
- SGD:S000004968
- Feature Type
- ORF , Verified
- Description
- Cytoplasmic RING-finger E3 Ub-ligase and sensor of stalled ribosomes; binds to Fpr1p and Smu2p, and triggers K63-linked polyubiquitination of monoubiquitinated 40S subunit Rps3p, from decoding-defective ribosomes, followed by dissociation of the 80S ribosomes and non-functional rRNA decay (NRD) of 18S rRNA; confers rapamycin resistance by competing with rapamycin for Fpr1p binding; accumulates in the nucleus in rapamycin-treated cells; ortholog of D. melanogaster shuttle craft and human NFX1 1 2
- Name Description
- FKBP12-Associated Protein 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Fap1p is 965 amino acids long, shorter-lived, low in abundance; contains 5 NF-X1-type zinc fingers; shares domains with proteins involved in protein modification and catalysis; ubiquitinylated on K340, phosphorylated on 5 residues
- Length (a.a.)
- 965
- Mol. Weight (Da)
- 108515.1
- Isoelectric Point
- 7.91
- Median Abundance (molecules/cell)
- 1091 +/- 649
- Half-life (hr)
- 7.1
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all FAP1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Cytoplasmic zinc- and protein-binding protein involved in nonfunctional rRNA decay
View computational annotations
Molecular Function
- Manually Curated
- enables protein binding (IDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in nonfunctional rRNA decay (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null displays chromosome instability, increased competitive fitness, small defect in vacuolar fragmentation, decreased utilization of glutamate as nitrogen source, decreased resistance to fluoride and to acids, increased accumulation of metals, decreased heat tolerance; overexpression increases resistance to rapamycin
Classical Genetics
- overexpression
- null
- null
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chromosome/plasmid maintenance: decreased
- competitive fitness: increased
- innate thermotolerance: decreased
- resistance to chemicals: decreased
- stress resistance: increased
- toxin resistance: decreased
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- viable
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Fap1p interacts physically with proteins involved in translation; FAP1 interacts genetically with genes involved in mitochondrion organization
176 total interactions for 150 unique genes
Physical Interactions
- Affinity Capture-MS: 89
- Affinity Capture-RNA: 2
- Co-localization: 2
- Co-purification: 1
- Protein-peptide: 1
- Two-hybrid: 5
Genetic Interactions
- Dosage Rescue: 1
- Negative Genetic: 63
- Positive Genetic: 10
- Synthetic Growth Defect: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-07-01
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.