MCK1 / YNL307C Overview
- Standard Name
- MCK1 1
- Systematic Name
- YNL307C
- SGD ID
- SGD:S000005251
- Aliases
- CMS1 1 24 , YPK1 25
- Feature Type
- ORF , Verified
- Description
- Dual-specificity S/T and tyrosine protein kinase; spindle position checkpoint component that works in parallel with Kin4p to prevent mitotic exit network (MEN) activation by the Cdc14 early anaphase release (FEAR) network; roles in chromosome segregation, meiotic entry, genome stability, phosphorylation-dependent degradation of Rcn1p and Cdc6p, inhibition of protein kinase A, transcriptional regulation, inhibition of RNAPIII, calcium stress and inhibition of Clb2p-Cdc28p after nuclear division 1 2 3 4 5 6 7 8 9
- Name Description
- Meiosis and Centromere regulatory Kinase 1
- Paralog
- YGK3 9
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- MCK1 has a paralog, YGK3, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 375
- Mol. Weight (Da)
- 43142.3
- Isoelectric Point
- 8.88
- Median Abundance (molecules/cell)
- 8487 +/- 3428
- Half-life (hr)
- 8.1
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all MCK1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein serine/threonine/tyrosine kinase involved in protein phosphorylation and breakdown; cyclin-binding protein; implicated in double-strand break repair via nonhomologous end joining, meiotic nuclear division, and mitotic chromosome segregation; involved in in ascospore formation and the cellular response to heat and salt stress
View computational annotations
Molecular Function
- Manually Curated
- enables cyclin binding (IPI)
- enables enzyme inhibitor activity (IMP)
- enables protein kinase activity (HDA)
- enables protein serine/threonine kinase activity (IDA)
- enables protein serine/threonine/tyrosine kinase activity (IDA)
Biological Process
- Manually Curated
- involved in ascospore formation (IMP)
- involved in double-strand break repair via nonhomologous end joining (IMP)
- involved in meiotic cell cycle (IMP)
- involved in mitotic sister chromatid segregation (IMP)
- acts upstream of or within mitotic spindle orientation checkpoint signaling (IGI)
- involved in protein catabolic process (IGI, IMP)
Cellular Component
- Manually Curated
- is active in cellular component unknown (ND)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant has a decreased growth rate, is thermosensitive and has reduced innate thermotolerance; null displays elevated rates of mitotic chromosome loss at semi-permissive temperatures and in the presence of benomyl; homozygous null mutant has delayed and reduced sporulation, with defects in ascus maturation; null mutant has a reduced critical cell size at START, delayed mitotic exit and elevated Clb2p-Cdc28p activity; null mutant is sensitive to DNA damaging agents, ionic, oxidative, and hyperosmotic stress; heterozygous null is haploinsufficient; overexpression increases filamentous growth and results in bud elongation
Classical Genetics
- null
- cell cycle progression in telophase: delayed
- chemical compound accumulation: decreased
- cold sensitivity: increased
- colony sectoring: increased
- critical cell size at START (G1 cell-size checkpoint): decreased
- ionic stress resistance: decreased
- metal resistance: decreased
- protein activity: increased
- protein/peptide accumulation: delayed
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- protein/peptide modification: delayed
- protein/peptide modification: increased
- resistance to chemicals: decreased
- RNA accumulation: decreased
- spore wall formation: abnormal
- sporulation efficiency: decreased
- sporulation: delayed
- overexpression
- null
- anaerobic growth: decreased
- auxotrophy
- cell size: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: increased
- chronological lifespan: decreased
- competitive fitness: decreased
- competitive fitness: increased
- desiccation resistance: decreased
- haploinsufficient
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- innate thermotolerance: decreased
- killer toxin resistance: decreased
- metal resistance: decreased
- nutrient utilization: decreased
- oxidative stress resistance: decreased
- oxidative stress resistance: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- sporulation: decreased
- toxin resistance: decreased
- toxin resistance: increased
- transposable element transposition: decreased
- UV resistance: decreased
- vegetative growth: abnormal
- vegetative growth: decreased rate
- viable
- overexpression
- reduction of function
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The mck1 null mutant is viable; the null mutant of paralog ygk3 is viable; the mck1 ygk3 double mutant displays a synthetic growth defect.
941 total interactions for 632 unique genes
Physical Interactions
- Affinity Capture-MS: 70
- Affinity Capture-RNA: 8
- Affinity Capture-Western: 7
- Biochemical Activity: 119
- Co-localization: 1
- Co-purification: 1
- PCA: 2
- Proximity Label-MS: 2
- Reconstituted Complex: 7
- Two-hybrid: 8
Genetic Interactions
- Dosage Growth Defect: 38
- Dosage Lethality: 5
- Dosage Rescue: 20
- Negative Genetic: 433
- Phenotypic Enhancement: 13
- Phenotypic Suppression: 5
- Positive Genetic: 50
- Synthetic Growth Defect: 115
- Synthetic Lethality: 18
- Synthetic Rescue: 19
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2000-10-02
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.