WTM1 / YOR230W Overview
- Standard Name
- WTM1 1
- Systematic Name
- YOR230W
- SGD ID
- SGD:S000005756
- Feature Type
- ORF , Verified
- Description
- Transcriptional modulator; involved in regulation of meiosis, silencing, and expression of RNR genes; acts as a nuclear anchor to retain the ribonucleotide reductase small subunit heterodimer, Rnr2p-Rnr4p, in the nucleus when cells are not in S phase; contains WD repeats 1 2 3
- Name Description
- WD repeat containing Transcriptional Modulator 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 437
- Mol. Weight (Da)
- 48367.0
- Isoelectric Point
- 5.0
- Median Abundance (molecules/cell)
- 21883 +/- 7927
- Half-life (hr)
- 15.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all WTM1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Transcription factor that regulates meiosis, chromatin silencing; inhibits ribonucleotide reductase by sequestering it in the nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables protein binding (IPI)
- enables transcription corepressor activity (IDA)
Biological Process
- Manually Curated
- involved in maintenance of protein location in nucleus (IMP)
- involved in meiotic cell cycle (IGI)
- involved in negative regulation of silent mating-type cassette heterochromatin formation (IGI)
- involved in negative regulation of transcription by RNA polymerase II (IGI)
- involved in positive regulation of transcription by RNA polymerase II (IMP, IDA, IGI)
- involved in protein import into nucleus (IPI, IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutants have abnormal vacuolar morphology, show increased competitive fitness in minimal medium; heterozygous diploid nulls are haploinsufficent and sensitive to starvation
Classical Genetics
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
148 total interactions for 110 unique genes
Physical Interactions
- Affinity Capture-MS: 55
- Affinity Capture-RNA: 3
- Affinity Capture-Western: 11
- Biochemical Activity: 1
- Co-localization: 3
- Co-purification: 3
- PCA: 1
- Proximity Label-MS: 1
- Two-hybrid: 6
Genetic Interactions
- Dosage Lethality: 1
- Dosage Rescue: 1
- Negative Genetic: 48
- Phenotypic Enhancement: 3
- Phenotypic Suppression: 1
- Positive Genetic: 8
- Synthetic Growth Defect: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- WTM1 encodes a transcriptional modulator of the WD40 repeat family. Wtm1p and its paralog Wtm2p act as both positive and negative regulators, depending on context. They positively regulate silenced genes at HM loci and telomeres, but negatively regulate the meiotic gene IME2 and ribonucleotide reductase RNR1. In the case of the RNR2, RNR3, and RNR4 ribonucleotide reductase genes, WTM1 and WTM2 amplify expression in response to inhibited replication. Wtm1p and Wtm2p may participate in chromatin remodeling to regulate transcription at other loci. Under normal growth conditions, Wtm1p acts as a nuclear anchor of the small ribonucleotide reductase subunit encoded by RNR2 and RNR4, thereby inhibiting activity of the RNR holoenzyme. Telomere length maintenance is modulated by Gln3p by its regulation of the expression of WTM1. WTM1 is post-transcriptionally down-regulated through binding of the zinc-finger proteins Tis11p and Cth1p to adenosine/uridine-rich elements (AU-rich elements or AREs) present in the 3' untranslated region (UTR) of its mRNA. In iron-limiting conditions, Tis11p and Cth1p promote degradation of WTM1 mRNA, decreasing levels of Wtm1p, allowing Rnr2p and Rnr2p to leave the nucleus, thereby facilitating assembly of an active ribonucleotide reductase enzyme in the cytoplasm, which promotes deoxyribonucleotide synthesis.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.