NCR1 / YPL006W Overview
- Standard Name
- NCR1 1
- Systematic Name
- YPL006W
- SGD ID
- SGD:S000005927
- Feature Type
- ORF , Verified
- Description
- Vacuolar membrane protein involved in sterol trafficking with Npc2p; conformational changes drive sterol translocation across the glycocalyx and into the vacuolar membrane; involved in sphingolipid metabolism; cells lacking Ncr1p exhibit high levels of long chain bases (LCBs), similar to accumulation of lipids observed in patients with Neimann-Pick C disease due to loss-of-function mutations in human NPC1; transits through the vacuolar protein sorting pathway via the AP-3 pathway 1 2 3 4 5 6
- Name Description
- Niemann-pick type C Related 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 1170
- Mol. Weight (Da)
- 132616.8
- Isoelectric Point
- 4.83
- Median Abundance (molecules/cell)
- 1907 +/- 1026
- Half-life (hr)
- 6.1
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all NCR1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sterol-binding protein involved in sphingolipid metabolism and sterol transport; localizes to vacuole membrane and endoplasmic reticulum
View computational annotations
Molecular Function
- Manually Curated
- enables sterol binding (IDA)
Biological Process
- Manually Curated
- involved in sphingolipid metabolic process (IMP)
- involved in sterol transport (IDA)
Cellular Component
- Manually Curated
- located in endoplasmic reticulum (HDA)
- located in fungal-type vacuole membrane (HDA, IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant accumulates phytosphingosine and phytosphingosine 1-phosphate, and shows abnormal mitochondria, respiratory defficiency and sensitivity to oxidative stress; a gain-of-function mutation causes resistance to aureobasidin A and edelfosine, and increased sensitivity to sphingosine
Classical Genetics
- null
- autophagy: absent
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: decreased
- mitochondrial morphology: abnormal
- oxidative stress resistance: decreased
- protein activity: decreased
- protein/peptide modification: decreased
- replicative lifespan: increased
- resistance to chemicals: increased
- respiratory growth: absent
- respiratory metabolism: decreased
- respiratory metabolism: decreased rate
- RNA accumulation: increased
- vegetative growth: decreased
- viable
- gain of function
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast NCR1 is homologous to human NPC1, and has been used to study Niemann-Pick disease
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
261 total interactions for 235 unique genes
Physical Interactions
- Affinity Capture-MS: 15
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 4
- Biochemical Activity: 1
- Co-localization: 1
- PCA: 4
- Protein-RNA: 1
- Reconstituted Complex: 3
- Two-hybrid: 14
Genetic Interactions
- Negative Genetic: 160
- Phenotypic Enhancement: 2
- Phenotypic Suppression: 4
- Positive Genetic: 36
- Synthetic Growth Defect: 4
- Synthetic Rescue: 6
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 8
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.