CIP1 / YPL014W Overview
- Standard Name
- CIP1 1
- Systematic Name
- YPL014W
- SGD ID
- SGD:S000005935
- Feature Type
- ORF , Verified
- Description
- Cyclin-dependent kinase inhibitor; activated by environmental stress; interacts with and inhibits the Cdc28p/Cln2p, G1/S phase cyclin-dependent kinase complex but not S-phase, or M-phase complexes; overexpression blocks cells in G1 phase and stabilizes the Cdc28p inhibitor Sic1p, while disruption accelerates the G1/S phase transition; phosphorylated during S phase in a Cdc28p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus 1 2 3
- Name Description
- Cdk1 Interacting Protein 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 381
- Mol. Weight (Da)
- 42483.0
- Isoelectric Point
- 9.12
- Median Abundance (molecules/cell)
- 3054 +/- 897
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CIP1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Inhibitor of the Cdc28p/Cln2p cyclin-dependent kinase complex; negative regulator of the G1/S phase transition; localizes to the nucleus and cytoplasm in a large-scale study
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in G1/S transition of mitotic cell cycle (IGI, IMP)
- involved in negative regulation of protein phosphorylation (IMP, IGI)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutation speeds up cell cycle progression through G1/S phase transition and causes decreased cell size; overexpression arrests G1/S phase transition; in systematic studies mutants show reduced competitive fitness and increased sensitivity to hyperosmotic stress
Classical Genetics
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
84 total interactions for 75 unique genes
Physical Interactions
- Affinity Capture-MS: 7
- Affinity Capture-RNA: 7
- Affinity Capture-Western: 5
- Biochemical Activity: 2
- Co-localization: 1
- Reconstituted Complex: 1
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Rescue: 2
- Negative Genetic: 45
- Positive Genetic: 11
- Synthetic Growth Defect: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- CIP1 is a cell cycle-regulated gene mainly expressed at G1, similar to that of G1 cyclin CLN2. CIP1 has two early cell cycle box (ECB) elements located at nucleotide −355 to −340 and −281 to −266 relative to the start codon. Mcm1p regulates CIP1 expression periodically at M/G1 transition through directly binding to the CIP1 promoter, which peaks at late mitosis. CIP1 expression is induced under osmotic stress, oxidative stress, carbon source starvation, DNA damage, heat shock, and rapamycin treatment. Stress-induced expression of CIP1 is driven by Msn2p and Msn4p via two STREs (stress response element).
- Regulators
- 54
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.