GCR1 / YPL075W Overview
- Standard Name
- GCR1 1
- Systematic Name
- YPL075W
- SGD ID
- SGD:S000005996
- Aliases
- LPF10 , SIT3 13
- Feature Type
- ORF , Verified
- Description
- Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p; contains a long intron, which allows multiple RNA and protein isoforms with varying expression levels depending on glucose availability 2 3 4
- Name Description
- GlyColysis Regulation 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 785
- Mol. Weight (Da)
- 87734.7
- Isoelectric Point
- 8.56
- Median Abundance (molecules/cell)
- 1002 +/- 528
- Half-life (hr)
- 9.9
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all GCR1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence-specific DNA binding RNA polymerase II transcription factor involved nucleosome organization, cellular response to glucose and the regulation of glycolysis, and the regulation of ribosomal protein gene expression; localizes to the nuclear envelope
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in negative regulation of transcription by RNA polymerase II (IMP)
- involved in nucleosome organization (IMP)
- involved in positive regulation of ribosomal protein gene transcription by RNA polymerase II (IMP)
- involved in positive regulation of transcription by RNA polymerase II (IMP)
- involved in positive regulation of transcription from a mobile element promoter (IMP)
- involved in regulation of glycolytic process (IMP)
Cellular Component
- Manually Curated
- located in nuclear envelope (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene, though systematic screen reported knockout as inviable; null mutants grow slowly, have large cells and increased respiration in the presence of repressing sugars; null also has decreased ethanol and acid tolerance, is sensitive to antifungals and DNA-damaging agent MMS
Classical Genetics
- null
- overexpression
- null
- reduction of function
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
122 total interactions for 97 unique genes
Physical Interactions
- Affinity Capture-MS: 5
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 8
- Reconstituted Complex: 2
- Two-hybrid: 12
Genetic Interactions
- Dosage Rescue: 3
- Phenotypic Enhancement: 5
- Phenotypic Suppression: 4
- Synthetic Lethality: 74
- Synthetic Rescue: 5
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- GCR1 encodes a leucine-zipper transcriptional activator of glycolytic and ribosomal protein genes, the two largest and most abundantly transcribed classes of glucose-induced target genes in yeast. Gcr1p is a DNA-binding protein which binds the consensus sequence 5'-TGGAAGCC-3', and the ability of Gcr1p to activate transcription of most of its target genes is entirely dependent upon Rap1p binding upstream of those genes. Genes with a Rap1p binding site in their promoter tend to be Gcr1p target genes. Gcr1p homodimerization is required for the Gcr1p contribution to the activation of Rap1p target genes. Gcr1p contributes to nutrient sensing and appears to have a role in repressing the transcription of glucose-repressed genes. Gcr1p and Rap1p both exhibit perinuclear localization and activation of target genes occurs at the nuclear periphery.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2005-07-21
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.