HRR25 / YPL204W Overview
- Standard Name
- HRR25 1
- Systematic Name
- YPL204W
- SGD ID
- SGD:S000006125
- Aliases
- KTI14 12 13
- Feature Type
- ORF , Verified
- Description
- Conserved casein kinase, monopolin subunit; regulates diverse events including vesicular traffic, DNA repair, CVT pathway, cohesion cleavage, attachment of sister kinetochores at meiosis I, exit from meiosis II, and ribosomal subunit biogenesis; binds the RNAPII CTD; phosphorylates COPII coat subunits; interacts with Sit4p phosphatase; antagonizes calcineurin signaling, reducing nuclear accumulation of Crz1p; phosphorylates Dsn1p; homolog of mammalian CK1delta 2 3 4 5 6 7 8 9 10 11
- Name Description
- HO and Radiation Repair 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 494
- Mol. Weight (Da)
- 57354.9
- Isoelectric Point
- 9.66
- Median Abundance (molecules/cell)
- 5798 +/- 1727
- Half-life (hr)
- 6.6
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all HRR25 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Dual-specificity serine/threonine and tyrosine protein kinase that regulates various cellular processes including clathrin-mediated endocytosis, ER to Golgi vesicle-mediated transport, pexophagy, ribosome maturation, and the attachment of microtubules to kinetochores; subunit of monopolin complex; also localizes to different sites within the cell, including bud necks and tips, plasma membrane, Golgi, and spindle pole bodies
View computational annotations
Molecular Function
- Manually Curated
- enables protein kinase activity (HDA)
- enables protein serine/threonine kinase activity (IMP, IDA)
- enables protein tyrosine kinase activity (IDA)
Biological Process
- Manually Curated
- involved in DNA repair (IMP)
- involved in pexophagy (IMP)
- involved in positive regulation of clathrin-dependent endocytosis (IMP)
- involved in regulation of autophagosome assembly (IMP)
- involved in regulation of ER to Golgi vesicle-mediated transport (IMP, IDA)
- involved in regulation of protein localization (IMP)
- involved in regulation of protein localization by the Cvt pathway (IMP)
- involved in regulation of vesicle fusion with Golgi apparatus (IMP)
- involved in ribosomal large subunit biogenesis (IMP)
- involved in ribosomal small subunit biogenesis (IDA, IMP)
- involved in spindle attachment to meiosis I kinetochore (IMP)
- involved in tRNA wobble uridine modification (IMP)
Cellular Component
- Manually Curated
- located in cellular bud neck (HDA, IDA)
- located in cellular bud tip (IDA)
- located in chromosome, centromeric region (IDA)
- located in cytoplasm (HDA)
- located in cytosol (HDA)
- located in Golgi apparatus (IDA)
- part of monopolin complex (IDA, IMP, IPI)
- located in nucleus (IDA)
- located in P-body (IDA)
- located in plasma membrane (IDA)
- located in spindle pole body (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene in S288C, non-essential in W303 and some other strain backgrounds; reduction-of-function mutations cause slow growth, abnormal cellular morphology, sensitivity to heat, X rays, killer toxins, caffeine, Calcofluor White and MMS, and increased production and excretion of inositol (Opi-); diploids are unable to sporulate
Classical Genetics
- repressible
- unspecified
- null
- reduction of function
- conditional
- dominant negative
- null
- repressible
- overexpression
- conditional
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast HRR25 is homologous to human CSNK1A1, CSNK1D, and CASK, and has been used to study familial adenomatous polyposis, cancer, brain diseases, neurodegenerative diseases, Alzheimer's disease, familial advanced sleep phase syndrome, and syndromic X-linked intellectual disability Najm type
Manually Curated
High-Throughput
- is_implicated_in syndromic X-linked intellectual disability Najm type (IGI, ISS)
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
877 total interactions for 631 unique genes
Physical Interactions
- Affinity Capture-MS: 272
- Affinity Capture-RNA: 9
- Affinity Capture-Western: 38
- Biochemical Activity: 44
- Co-crystal Structure: 1
- Co-fractionation: 1
- Co-purification: 2
- PCA: 1
- Proximity Label-MS: 1
- Reconstituted Complex: 46
- Two-hybrid: 8
Genetic Interactions
- Dosage Growth Defect: 2
- Dosage Lethality: 3
- Dosage Rescue: 2
- Negative Genetic: 382
- Phenotypic Enhancement: 2
- Phenotypic Suppression: 11
- Positive Genetic: 39
- Synthetic Growth Defect: 6
- Synthetic Rescue: 7
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.