SNF1 / YDR477W Overview
- Standard Name
- SNF1 1
- Systematic Name
- YDR477W
- SGD ID
- SGD:S000002885
- Aliases
- CAT1 56 , CCR1 58 , GLC2 66 , HAF3 67 , PAS14 68
- Feature Type
- ORF , Verified
- Description
- AMP-activated S/T protein kinase (AMPK); complexes with Snf4p and a Sip1p/Sip2p/Gal83p family member; required for glucose-shortage induced respiratory gene expression, heat shock, sporulation, and peroxisome biogenesis; regulates filamentous growth; acts as a non-canonical GEF activating Arf3p during invasive growth; role in mitotic spindle alignment in non-limiting glucose; phosphorylates Kcs1p; target of SUMO-dependent, Ub-mediated destruction; polyhistidine tract acts as a pH sensing module 2 3 5 6 7 8 9 10 11
- Name Description
- Sucrose NonFermenting 4
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Snf1p is 633 amino acids long, of moderate half-life, and low-to-moderate abundance; acetylated on K59 and K68, ubiquitinylated on 6 lysines, phosphorylated on 31 residues
- Length (a.a.)
- 633
- Mol. Weight (Da)
- 72033.1
- Isoelectric Point
- 6.65
- Median Abundance (molecules/cell)
- 5622 +/- 2394
- Half-life (hr)
- 9.2
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SNF1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- AMP-activated serine/threonine kinase subunit of the AMP-activated protein kinase complex; also serves as guanine nucleotide exchange factor (GEF) specific for Arf3p in response to glucose depletion; regulates both pseudohyphal and filamentous growth in response to starvation and carbohydrate metabolic process; inhibits translation of GCN4; involved in mitotic spindle alignment along the mother-bud axis
View computational annotations
Molecular Function
- Manually Curated
- enables AMP-activated protein kinase activity (IMP, IDA)
- enables guanyl-nucleotide exchange factor activity (IDA)
- enables protein kinase activity (HDA)
- enables protein serine/threonine kinase activity (IDA, IMP)
Biological Process
- Manually Curated
- involved in cellular response to glucose starvation (IDA)
- involved in cellular response to stress (IMP)
- involved in establishment of mitotic spindle orientation (IMP, IGI)
- involved in fungal-type cell wall assembly (IMP)
- involved in invasive growth in response to glucose limitation (IMP)
- involved in negative regulation of inositol phosphate biosynthetic process (IMP)
- involved in negative regulation of translation (IMP, IGI)
- involved in positive regulation of filamentous growth of a population of unicellular organisms in response to starvation (IMP)
- involved in positive regulation of gluconeogenesis (IMP)
- involved in positive regulation of macroautophagy (IMP)
- involved in positive regulation of pseudohyphal growth (IGI, IMP)
- involved in response to endoplasmic reticulum stress (IMP)
- involved in response to unfolded protein (IMP)
- involved in single-species surface biofilm formation (IMP)
Cellular Component
- Manually Curated
- located in cellular bud neck septin ring (IDA)
- located in cytoplasm (IDA)
- located in mitochondrion (HDA)
- located in nuclear envelope lumen (IDA)
- part of nucleotide-activated protein kinase complex (IPI, IDA)
- located in nucleus (IDA)
- located in vacuolar membrane (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; mutants are defective in glucose-dependent regulation of transcription, with pleiotropic effects on carbon source utilization, respiratory growth, cell wall structure, thermotolerance, hyperosmotic stress and starvation sensitivity, autophagy, peroxisome biogenesis, filamentous growth, sporulation in diploids
Classical Genetics
- reduction of function
- null
- alkaline pH resistance: decreased
- apoptosis: increased
- autophagy: decreased
- auxotrophy
- cell wall morphology: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: decreased
- cold sensitivity: increased
- desiccation resistance: absent
- desiccation resistance: decreased
- endoplasmic reticulum distribution: decreased
- entry into G0 (stationary phase): decreased
- Golgi distribution: increased
- hyperosmotic stress resistance: decreased
- invasive growth: decreased
- lipid particle morphology: abnormal
- metal resistance: decreased
- oxidative stress resistance: decreased
- protein activity: decreased
- protein/peptide accumulation: decreased
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- protein/peptide modification: absent
- protein/peptide modification: decreased
- protein/peptide modification: increased
- pseudohyphal growth: absent
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- respiratory growth: absent
- respiratory metabolism: decreased
- respiratory metabolism: increased
- spindle morphology: abnormal
- utilization of carbon source: absent
- utilization of carbon source: decreased
- vegetative growth: decreased
- overexpression
- conditional
- gain of function
- activation
- null
- acid pH resistance: decreased
- biofilm formation: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chronological lifespan: decreased
- competitive fitness: decreased
- desiccation resistance: decreased
- endoplasmic reticulum morphology: abnormal
- freeze-thaw resistance: decreased
- haploinsufficient
- heat sensitivity: increased
- hydrostatic pressure resistance: decreased
- hyperosmotic stress resistance: decreased
- mating response: decreased
- metal resistance: decreased
- protein activity: increased
- protein/peptide accumulation: decreased
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- resistance to enzymatic treatment: decreased
- respiratory growth: absent
- respiratory growth: decreased rate
- sporulation efficiency: decreased
- sporulation: absent
- stress resistance: increased
- toxin resistance: increased
- utilization of carbon source: decreased
- utilization of carbon source: decreased rate
- utilization of carbon source: increased
- utilization of nitrogen source: decreased rate
- vegetative growth: absent
- vegetative growth: decreased rate
- viable
- overexpression
- reduction of function
- anaerobic growth: increased
- heat sensitivity: increased
- osmotic stress resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: decreased
- respiratory growth: increased
- starvation resistance: increased
- utilization of carbon source: decreased
- vegetative growth: increased
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Snf1p interacts physically with proteins involved in transcription; SNF1 interacts genetically with genes involved in transcription
1434 total interactions for 848 unique genes
Physical Interactions
- Affinity Capture-MS: 141
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 69
- Biochemical Activity: 127
- Co-crystal Structure: 2
- Co-fractionation: 1
- Co-localization: 6
- Co-purification: 2
- PCA: 5
- Reconstituted Complex: 42
- Two-hybrid: 92
Genetic Interactions
- Dosage Growth Defect: 8
- Dosage Lethality: 1
- Dosage Rescue: 36
- Negative Genetic: 627
- Phenotypic Enhancement: 26
- Phenotypic Suppression: 44
- Positive Genetic: 77
- Synthetic Growth Defect: 46
- Synthetic Lethality: 9
- Synthetic Rescue: 69
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2006-03-27
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.