TOM70 / YNL121C Overview
- Standard Name
- TOM70 1
- Systematic Name
- YNL121C
- SGD ID
- SGD:S000005065
- Aliases
- MAS70 28 , MOM72 29 , OMP1
- Feature Type
- ORF , Verified
- Description
- Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially-directed proteins, acting as a receptor for incoming precursor proteins; moonlighting role as a transcriptional regulator of mitochondrial proteins; age-dependent reduction in Tom70p results in mitochondrial dysfunction, a hallmark of aging; localizes to mitochondria–ER contact sites; TOM70 has a paralog, TOM71, that arose from the whole genome duplication 2 3 4 5 6 7 8
- Name Description
- Translocase of the Outer Mitochondrial membrane 1
- Paralog
- TOM71 6
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- TOM70 has a paralog, TOM71, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 617
- Mol. Weight (Da)
- 70110.7
- Isoelectric Point
- 4.97
- Median Abundance (molecules/cell)
- 14978 +/- 9530
- Half-life (hr)
- 10.1
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all TOM70 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein transmembrane transporter with mitochondrion targeting sequence binding activity involved in protein targeting to mitochondrion, protein import into mitochondrial matrix, and in protein insertion into mitochondrial inner membrane; subunit of mitochondrial outer membrane translocase complex
View computational annotations
Molecular Function
- Manually Curated
- enables mitochondrion targeting sequence binding (IMP)
- contributes to protein transmembrane transporter activity (IMP)
Biological Process
- Manually Curated
- involved in protein import into mitochondrial matrix (IMP)
- involved in protein insertion into mitochondrial inner membrane (IMP, IDA)
- involved in protein targeting to mitochondrion (IGI)
Cellular Component
- Manually Curated
- is active in mitochondria-associated endoplasmic reticulum membrane contact site (IDA)
- located in mitochondrial outer membrane (HDA, IDA)
- part of mitochondrial outer membrane translocase complex (IDA)
- located in mitochondrion (HDA, IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant is petite-negative (loss of the mitochondrial genome is lethal), has a respiratory growth defect that is exacerbated at high temperature, and exhibits slow growth on fermentable carbon sources; null mutant shows increased replicative lifespan extension in response to caloric restriction, compared to wild type; in large-scale studies, null mutant has reduced competitive fitness in various media, decreased invasive growth, biofilm formation, and resistance to starvation and desiccation, and shows altered resistance to various chemicals
Classical Genetics
- null
- heat sensitivity: increased
- mitochondrial genome maintenance: abnormal
- mitochondrial transport: decreased rate
- petite-negative
- protein/peptide accumulation: decreased
- replicative lifespan: increased
- respiratory growth: absent
- respiratory growth: decreased rate
- respiratory metabolism: absent
- toxin resistance: decreased
- vegetative growth: decreased rate
- viable
- overexpression
- repressible
- null
- biofilm formation: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: increased
- chronological lifespan: increased
- colony appearance: abnormal
- competitive fitness: decreased
- desiccation resistance: decreased
- haploinsufficient
- heat sensitivity: increased
- invasive growth: absent
- petite-negative
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- starvation resistance: decreased
- stress resistance: increased
- vacuolar morphology: abnormal
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The tom70 null mutant is viable; the null mutant of paralog tom71 is viable; the tom70 tom71 double mutant displays a synthetic growth defect.
578 total interactions for 401 unique genes
Physical Interactions
- Affinity Capture-MS: 42
- Affinity Capture-RNA: 9
- Affinity Capture-Western: 27
- Biochemical Activity: 3
- Co-fractionation: 15
- Co-localization: 2
- Co-purification: 4
- Cross-Linking-MS (XL-MS): 4
- FRET: 1
- PCA: 11
- Proximity Label-MS: 1
- Reconstituted Complex: 24
- Two-hybrid: 1
Genetic Interactions
- Dosage Growth Defect: 2
- Dosage Rescue: 25
- Negative Genetic: 348
- Phenotypic Enhancement: 4
- Phenotypic Suppression: 3
- Positive Genetic: 20
- Synthetic Growth Defect: 20
- Synthetic Lethality: 7
- Synthetic Rescue: 5
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 7
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2009-03-24
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.