TSC10 / YBR265W Overview
- Standard Name
- TSC10 1
- Systematic Name
- YBR265W
- SGD ID
- SGD:S000000469
- Feature Type
- ORF , Verified
- Description
- 3-ketosphinganine reductase; catalyzes the second step in phytosphingosine synthesis; essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine; localized to lipid droplets; member of short chain dehydrogenase/reductase protein family; mutations in human homolog KDSR cause recessive progressive symmetric erythrokeratoderma 1 2 3 4
- Name Description
- Temperature-sensitive Suppressors of Csg2 mutants 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 320
- Mol. Weight (Da)
- 35968.9
- Isoelectric Point
- 6.25
- Median Abundance (molecules/cell)
- 2283 +/- 549
- Half-life (hr)
- 4.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all TSC10 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- 3-ketosphinganine reductase of the chemical reactions and pathways involving 3-keto-dihydrosphingosine; also involved in the formation of sphingolipids; localizes to lipid particles, endoplasmic reticulum, and outer membranes of mitochondria
View computational annotations
Molecular Function
- Manually Curated
- enables 3-dehydrosphinganine reductase activity (IDA)
Biological Process
- Manually Curated
- involved in 3-keto-sphinganine metabolic process (IDA)
- involved in sphingolipid biosynthetic process (IMP, IDA)
Cellular Component
- Manually Curated
- located in cytoplasm (HDA)
- located in endoplasmic reticulum (HDA)
- located in lipid droplet (IDA)
- located in mitochondrial outer membrane (HDA)
Pathways
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; diploid heterozygous null mutant is sensitive to dihydromotuporamine C; in large-scale studies, repression confers lower competitive fitness, sensitivity to HU and MMS, and resistance to myriocin; diploid heterozygous null mutant is sensitive to a human cationic antimicrobial peptide
Classical Genetics
- null
- reduction of function
- repressible
- conditional
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast TSC10 is homologous to human KDSR, and has been used to study recessive progressive symmetric erythrokeratoderma characterized by severe lesions of thick scaly skin on face and genitals and thickened, red, and scaly skin on hands and feet
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
157 total interactions for 140 unique genes
Physical Interactions
- Affinity Capture-MS: 7
- Affinity Capture-RNA: 4
- Biochemical Activity: 1
- Co-localization: 1
- Co-purification: 1
- PCA: 1
- Proximity Label-MS: 1
- Reconstituted Complex: 1
Genetic Interactions
- Dosage Growth Defect: 2
- Negative Genetic: 108
- Phenotypic Suppression: 1
- Positive Genetic: 23
- Synthetic Lethality: 5
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 8
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2007-10-05
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.