MPS1 / YDL028C Overview
- Standard Name
- MPS1 1
- Systematic Name
- YDL028C
- SGD ID
- SGD:S000002186
- Aliases
- RPK1 21 , PAC8 24
- Feature Type
- ORF , Verified
- Description
- Dual-specificity kinase; required for SPB duplication and spindle checkpoint function in mitosis and meiosis; contributes to bi-orientation, promoting formation of force-generating kinetochore-microtubule attachments in meiosis I; autophosphorylation required for function; substrates include SPB proteins (Spc42p, Spc110p, Spc98p), mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p; substrate of APCC(Cdh1); detected in peroxisomes; similar to human Mps1p 2 3 4 5 6 7 8 9 10 11
- Name Description
- MonoPolar Spindle 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 764
- Mol. Weight (Da)
- 86783.9
- Isoelectric Point
- 9.36
- Median Abundance (molecules/cell)
- 536 +/- 526
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all MPS1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein serine/threonine/tyrosine kinase involved in the phosphorylation of many mitotic regulators; capable of autophosphorylation; required for spindle pole body duplication and the mitotic spindle assembly checkpoint; involved in sister chromatid biorientation in mitosis and meiosis; regulates the attachment of spindle microtubules to the kinetochore; localizes to the spindle pole body and the kinetochore
View computational annotations
Molecular Function
- Manually Curated
- enables kinetochore binding (IDA)
- enables protein kinase activity (IDA, HDA)
- enables protein serine/threonine kinase activity (IDA)
- enables protein serine/threonine/tyrosine kinase activity (IMP, IDA)
Biological Process
- Manually Curated
- acts upstream of or within meiotic spindle checkpoint signaling (IGI)
- acts upstream of or within mitotic spindle assembly checkpoint signaling (IGI, IMP)
- involved in protein localization to kinetochore (IGI)
- involved in regulation of attachment of spindle microtubules to kinetochore (IMP)
- involved in sister chromatid biorientation (IMP)
- involved in spindle assembly (IMP)
- involved in spindle pole body duplication (IMP)
Cellular Component
- Manually Curated
- located in kinetochore (IDA)
- located in peroxisome (IDA)
- located in spindle pole body (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; conditional mutant has abnormal spindle morphology with monopolar spindles, contains a single SPB with enlarged half-bridge, and becomes polyploid due to chromosome missegregation, resulting in decreased viability; overexpression results in decreased vegetative growth, with an accumulation of cells with medium to large buds in G2 phase; overexpression also results in increased invasive growth
Classical Genetics
- null
- unspecified
- overexpression
- repressible
- conditional
- chromosome segregation: abnormal
- chromosome/plasmid maintenance: abnormal
- growth in exponential phase: decreased
- heat sensitivity: increased
- polyploidy: increased
- protein/peptide accumulation: decreased
- protein/peptide distribution: abnormal
- spindle morphology: abnormal
- vegetative growth: decreased rate
- viability: decreased
- null
- overexpression
- reduction of function
- conditional
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast MPS1 is homologous to human GRK3, and has been used to study bipolar disorder
High-Throughput
- is_implicated_in bipolar disorder (IGI, ISS)
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
845 total interactions for 636 unique genes
Physical Interactions
- Affinity Capture-MS: 41
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 12
- Biochemical Activity: 35
- Co-localization: 1
- Co-purification: 2
- Protein-peptide: 1
- Reconstituted Complex: 25
- Two-hybrid: 10
Genetic Interactions
- Dosage Growth Defect: 3
- Dosage Lethality: 13
- Dosage Rescue: 9
- Negative Genetic: 536
- Phenotypic Enhancement: 2
- Phenotypic Suppression: 9
- Positive Genetic: 109
- Synthetic Growth Defect: 8
- Synthetic Lethality: 17
- Synthetic Rescue: 6
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2000-04-05
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.