CDC40 / YDR364C Overview
- Standard Name
- CDC40 1
- Systematic Name
- YDR364C
- SGD ID
- SGD:S000002772
- Aliases
- SLU4 5 , PRP17 6 , SLT15 7
- Feature Type
- ORF , Verified
- Description
- Pre-mRNA splicing factor; important for catalytic step II of pre-mRNA splicing and plays a role in cell cycle progression, particularly at the G1/S phase transition; required for DNA synthesis during mitosis and meiosis; has WD repeats; thermosensitivity of the cdc40 null mutant is functionally complemented by a chimeric construct containing the N-terminal 156 amino acids of yeast Cdc40p fused to the C-terminal two thirds (297 amino acids) of human CDC40 2 3 4
- Name Description
- Cell Division Cycle 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 455
- Mol. Weight (Da)
- 52045.4
- Isoelectric Point
- 7.22
- Median Abundance (molecules/cell)
- 1630 +/- 566
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CDC40 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein involved in mRNA splicing; contributes to the second transesterification reaction of the spliceosomal complex
View computational annotations
Molecular Function
- Manually Curated
- contributes to second spliceosomal transesterification activity (IMP)
Biological Process
- Manually Curated
- involved in generation of catalytic spliceosome for second transesterification step (IMP, IGI)
- involved in mRNA 3'-splice site recognition (IGI)
- involved in mRNA branch site recognition (IGI)
Cellular Component
- Manually Curated
- located in nuclear periphery (IDA)
- part of spliceosomal complex (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene in S288C, but essential in the Sigma1278b background; null mutant has slow growth in S288C, and is unable to grow anaerobically; conditional mutants accumulate splicing intermediates and both SPBs and mitotic spindles are absent; homozygous conditional mutants have deceased microtubule staining, defects in meiotic recombination and chromosome segregation and are sensitive to treatment with MMS; null mutants have cell cycle defects in G1 and S phase, abnormally elongated buds, delayed bud emergence, decreased chronological lifespan, reduced survival in stationary phase, and decreased spontaneous direct-repeat recombination
Classical Genetics
- null
- conditional
- null
- acid pH resistance: decreased
- bud morphology: abnormal
- cell size: increased
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: decreased
- endocytosis: decreased
- heat sensitivity: decreased
- inviable
- killer toxin resistance: decreased
- lipid particle morphology: abnormal
- metal resistance: decreased
- mitotic recombination: decreased
- prion formation: decreased
- resistance to chemicals: decreased
- resistance to enzymatic treatment: decreased
- respiratory growth: absent
- sporulation: decreased
- stress resistance: decreased
- transposable element transposition: increased
- utilization of carbon source: decreased
- utilization of nitrogen source: absent
- UV resistance: decreased
- vegetative growth: absent
- vegetative growth: decreased rate
- viable
- conditional
- reduction of function
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
286 total interactions for 189 unique genes
Physical Interactions
- Affinity Capture-MS: 71
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 7
- Co-fractionation: 1
- Co-localization: 1
- Co-purification: 2
- Reconstituted Complex: 7
- Two-hybrid: 2
Genetic Interactions
- Dosage Lethality: 2
- Dosage Rescue: 3
- Negative Genetic: 89
- Phenotypic Enhancement: 4
- Positive Genetic: 3
- Synthetic Growth Defect: 19
- Synthetic Lethality: 48
- Synthetic Rescue: 22
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 4
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.